DESCRIBE <http://purl.uniprot.org/citations/18840784>

Subject	Predicate	Object
http://purl.uniprot.org/citations/18840784	http://www.w3.org/1999/02/22-rdf-syntax-ns#type	http://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/18840784	http://www.w3.org/2000/01/rdf-schema#comment	" Objective There is evidence that insulin reduces brain injury evoked by ischemia/reperfusion (I/R). However, the molecular mechanisms underlying the protective effects of insulin remain unknown. Insulin is a well-known inhibitor of glycogen synthase kinase-3beta (GSK-3beta). Here, we investigate the role of GSK-3beta inhibition on I/R-induced cerebral injury in a rat model of insulinopenic diabetes. Research design and methods Rats with streptozotocin-induced diabetes were subjected to 30-min occlusion of common carotid arteries followed by 1 or 24 h of reperfusion. Insulin (2-12 IU/kg i.v.) or the selective GSK-3beta inhibitor TDZD-8 (0.2-3 mg/kg i.v.) was administered during reperfusion. Results Insulin or TDZD-8 dramatically reduced infarct volume and levels of S100B protein, a marker of cerebral injury. Both drugs induced phosphorylation of the Ser9 residue, thereby inactivating GSK-3beta in the rat hippocampus. Insulin, but not TDZD-8, lowered blood glucose. The hippocampi of the drug-treated animals displayed reduced oxidative stress at 1 h of reperfusion as shown by the decreased generation of reactive oxygen species and lipid peroxidation. I/R-induced activation of nuclear factor-kappaB was attenuated by both drug treatments. At 24 h of reperfusion, TDZD-8 and insulin significantly reduced plasma levels of tumor necrosis factor-alpha; neutrophil infiltration, measured as myeloperoxidase activity and intercellular-adhesion-molecule-1 expression; and cyclooxygenase-2 and inducible-NO-synthase expression. Conclusions Acute administration of insulin or TDZD-8 reduced cerebral I/R injury in diabetic rats. We propose that the inhibitory effect on the activity of GSK-3beta contributes to the protective effect of insulin independently of any effects on blood glucose."xsd:string
http://purl.uniprot.org/citations/18840784	http://purl.org/dc/terms/identifier	"doi:10.2337/db08-0691"xsd:string
http://purl.uniprot.org/citations/18840784	http://purl.uniprot.org/core/author	"Aragno M."xsd:string
http://purl.uniprot.org/citations/18840784	http://purl.uniprot.org/core/author	"Fantozzi R."xsd:string
http://purl.uniprot.org/citations/18840784	http://purl.uniprot.org/core/author	"Thiemermann C."xsd:string
http://purl.uniprot.org/citations/18840784	http://purl.uniprot.org/core/author	"Boccuzzi G."xsd:string
http://purl.uniprot.org/citations/18840784	http://purl.uniprot.org/core/author	"Collino M."xsd:string
http://purl.uniprot.org/citations/18840784	http://purl.uniprot.org/core/author	"Castiglia S."xsd:string
http://purl.uniprot.org/citations/18840784	http://purl.uniprot.org/core/author	"Tomasinelli C."xsd:string
http://purl.uniprot.org/citations/18840784	http://purl.uniprot.org/core/date	"2009"xsd:gYear
http://purl.uniprot.org/citations/18840784	http://purl.uniprot.org/core/name	"Diabetes"xsd:string
http://purl.uniprot.org/citations/18840784	http://purl.uniprot.org/core/pages	"235-242"xsd:string
http://purl.uniprot.org/citations/18840784	http://purl.uniprot.org/core/title	"Insulin reduces cerebral ischemia/reperfusion injury in the hippocampus of diabetic rats: a role for glycogen synthase kinase-3beta."xsd:string
http://purl.uniprot.org/citations/18840784	http://purl.uniprot.org/core/volume	"58"xsd:string
http://purl.uniprot.org/citations/18840784	http://www.w3.org/2004/02/skos/core#exactMatch	http://purl.uniprot.org/pubmed/18840784
http://purl.uniprot.org/citations/18840784	http://xmlns.com/foaf/0.1/primaryTopicOf	https://pubmed.ncbi.nlm.nih.gov/18840784
http://purl.uniprot.org/uniprot/#_A0A8I6A0X7-mappedCitation-18840784	http://www.w3.org/1999/02/22-rdf-syntax-ns#object	http://purl.uniprot.org/citations/18840784
http://purl.uniprot.org/uniprot/#_A0A8L2PZQ2-mappedCitation-18840784	http://www.w3.org/1999/02/22-rdf-syntax-ns#object	http://purl.uniprot.org/citations/18840784
http://purl.uniprot.org/uniprot/#_P04631-mappedCitation-18840784	http://www.w3.org/1999/02/22-rdf-syntax-ns#object	http://purl.uniprot.org/citations/18840784
http://purl.uniprot.org/uniprot/#_P18266-mappedCitation-18840784	http://www.w3.org/1999/02/22-rdf-syntax-ns#object	http://purl.uniprot.org/citations/18840784
http://purl.uniprot.org/uniprot/P04631	http://purl.uniprot.org/core/mappedCitation	http://purl.uniprot.org/citations/18840784
http://purl.uniprot.org/uniprot/P18266	http://purl.uniprot.org/core/mappedCitation	http://purl.uniprot.org/citations/18840784
http://purl.uniprot.org/uniprot/A0A8L2PZQ2	http://purl.uniprot.org/core/mappedCitation	http://purl.uniprot.org/citations/18840784
http://purl.uniprot.org/uniprot/A0A8I6A0X7	http://purl.uniprot.org/core/mappedCitation	http://purl.uniprot.org/citations/18840784