| http://purl.uniprot.org/citations/18844376 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18844376 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18844376 | http://www.w3.org/2000/01/rdf-schema#comment | "Monomeric Kusabira Orange (mKO) is a green fluorescent protein (GFP)-like protein that emits orange light at a peak of 559 nm. We analyzed its X-ray structure at 1.65 A and found a novel three-ring chromophore that developed autocatalytically from a Cys65-Tyr66-Glu67 tripeptide in which the side chain of Cys65 formed the third 2-hydroxy-3-thiazoline ring. As a result, the chromophore contained the CNCOH group at the 2-position of the imidazolinone moiety such that the conjugated pi-electron system of the chromophore was more extended than that of GFP but less extended than that of the Discosoma sp. red fluorescent protein (DsRed). Since a sulfur atom has potent nucleophilic character, the third 3-thiazoline ring is rapidly and completely cyclized. Furthermore, our structure reveals the presence of a pi-pi stacking interaction between His197 and the chromophore as well as a pi-cation interaction between Arg69 and the chromophore. These structural findings are sufficient to account for the orange emission, pH tolerance, and photostability of mKO."xsd:string |
| http://purl.uniprot.org/citations/18844376 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi800727v"xsd:string |
| http://purl.uniprot.org/citations/18844376 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi800727v"xsd:string |
| http://purl.uniprot.org/citations/18844376 | http://purl.uniprot.org/core/author | "Mizuno H."xsd:string |
| http://purl.uniprot.org/citations/18844376 | http://purl.uniprot.org/core/author | "Mizuno H."xsd:string |
| http://purl.uniprot.org/citations/18844376 | http://purl.uniprot.org/core/author | "Shiro Y."xsd:string |
| http://purl.uniprot.org/citations/18844376 | http://purl.uniprot.org/core/author | "Shiro Y."xsd:string |
| http://purl.uniprot.org/citations/18844376 | http://purl.uniprot.org/core/author | "Kikuchi A."xsd:string |
| http://purl.uniprot.org/citations/18844376 | http://purl.uniprot.org/core/author | "Kikuchi A."xsd:string |
| http://purl.uniprot.org/citations/18844376 | http://purl.uniprot.org/core/author | "Miyawaki A."xsd:string |
| http://purl.uniprot.org/citations/18844376 | http://purl.uniprot.org/core/author | "Miyawaki A."xsd:string |
| http://purl.uniprot.org/citations/18844376 | http://purl.uniprot.org/core/author | "Karasawa S."xsd:string |
| http://purl.uniprot.org/citations/18844376 | http://purl.uniprot.org/core/author | "Karasawa S."xsd:string |
| http://purl.uniprot.org/citations/18844376 | http://purl.uniprot.org/core/author | "Fukumura E."xsd:string |
| http://purl.uniprot.org/citations/18844376 | http://purl.uniprot.org/core/author | "Fukumura E."xsd:string |
| http://purl.uniprot.org/citations/18844376 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18844376 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18844376 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/18844376 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/18844376 | http://purl.uniprot.org/core/pages | "11573-11580"xsd:string |
| http://purl.uniprot.org/citations/18844376 | http://purl.uniprot.org/core/pages | "11573-11580"xsd:string |
| http://purl.uniprot.org/citations/18844376 | http://purl.uniprot.org/core/title | "Structural characterization of a thiazoline-containing chromophore in an orange fluorescent protein, monomeric Kusabira Orange."xsd:string |
| http://purl.uniprot.org/citations/18844376 | http://purl.uniprot.org/core/title | "Structural characterization of a thiazoline-containing chromophore in an orange fluorescent protein, monomeric Kusabira Orange."xsd:string |