| http://purl.uniprot.org/citations/18849488 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18849488 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18849488 | http://www.w3.org/2000/01/rdf-schema#comment | "We demonstrate that in zebrafish, the microRNA miR-451 plays a crucial role in promoting erythroid maturation, in part via its target transcript gata2. Zebrafish miR-144 and miR-451 are processed from a single precursor transcript selectively expressed in erythrocytes. In contrast to other hematopoietic mutants, the zebrafish mutant meunier (mnr) showed intact erythroid specification but diminished miR-144/451 expression. Although erythropoiesis initiated normally in mnr, erythrocyte maturation was morphologically retarded. Morpholino knockdown of miR-451 increased erythrocyte immaturity in wild-type embryos, and miR-451 RNA duplexes partially rescued erythroid maturation in mnr, demonstrating a requirement and role for miR-451 in erythrocyte maturation. mnr provided a selectively miR-144/451-deficient background, facilitating studies to discern miRNA function and validate candidate targets. Among computer-predicted miR-451 targets potentially mediating these biologic effects, the pro-stem cell transcription factor gata2 was an attractive candidate. In vivo reporter assays validated the predicted miR-451/gata2-3'UTR interaction, gata2 down-regulation was delayed in miR-451-knockdown and mnr embryos, and gata2 knockdown partially restored erythroid maturation in mnr, collectively confirming gata2 down-regulation as pivotal for miR-451-driven erythroid maturation. These studies define a new genetic pathway promoting erythroid maturation (mnr/miR-451/gata2) and provide a rare example of partial rescue of a mutant phenotype solely by miRNA overexpression."xsd:string |
| http://purl.uniprot.org/citations/18849488 | http://purl.org/dc/terms/identifier | "doi:10.1182/blood-2008-05-155812"xsd:string |
| http://purl.uniprot.org/citations/18849488 | http://purl.uniprot.org/core/author | "Waterhouse P.M."xsd:string |
| http://purl.uniprot.org/citations/18849488 | http://purl.uniprot.org/core/author | "Waterhouse P.M."xsd:string |
| http://purl.uniprot.org/citations/18849488 | http://purl.uniprot.org/core/author | "Kloosterman W.P."xsd:string |
| http://purl.uniprot.org/citations/18849488 | http://purl.uniprot.org/core/author | "Kloosterman W.P."xsd:string |
| http://purl.uniprot.org/citations/18849488 | http://purl.uniprot.org/core/author | "Lieschke G.J."xsd:string |
| http://purl.uniprot.org/citations/18849488 | http://purl.uniprot.org/core/author | "Lieschke G.J."xsd:string |
| http://purl.uniprot.org/citations/18849488 | http://purl.uniprot.org/core/author | "Layton J.E."xsd:string |
| http://purl.uniprot.org/citations/18849488 | http://purl.uniprot.org/core/author | "Layton J.E."xsd:string |
| http://purl.uniprot.org/citations/18849488 | http://purl.uniprot.org/core/author | "Carradice D."xsd:string |
| http://purl.uniprot.org/citations/18849488 | http://purl.uniprot.org/core/author | "Carradice D."xsd:string |
| http://purl.uniprot.org/citations/18849488 | http://purl.uniprot.org/core/author | "Pase L."xsd:string |
| http://purl.uniprot.org/citations/18849488 | http://purl.uniprot.org/core/author | "Pase L."xsd:string |
| http://purl.uniprot.org/citations/18849488 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/18849488 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/18849488 | http://purl.uniprot.org/core/name | "Blood"xsd:string |
| http://purl.uniprot.org/citations/18849488 | http://purl.uniprot.org/core/name | "Blood"xsd:string |
| http://purl.uniprot.org/citations/18849488 | http://purl.uniprot.org/core/pages | "1794-1804"xsd:string |
| http://purl.uniprot.org/citations/18849488 | http://purl.uniprot.org/core/pages | "1794-1804"xsd:string |
| http://purl.uniprot.org/citations/18849488 | http://purl.uniprot.org/core/title | "miR-451 regulates zebrafish erythroid maturation in vivo via its target gata2."xsd:string |
| http://purl.uniprot.org/citations/18849488 | http://purl.uniprot.org/core/title | "miR-451 regulates zebrafish erythroid maturation in vivo via its target gata2."xsd:string |
| http://purl.uniprot.org/citations/18849488 | http://purl.uniprot.org/core/volume | "113"xsd:string |