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http://purl.uniprot.org/citations/18928376http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/18928376http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/18928376http://www.w3.org/2000/01/rdf-schema#comment"

Background

We previously reported that group A Streptococcus (GAS) pili are the T antigens described by Rebecca Lancefield. We also showed that these pili, constituted by backbone, ancillary 1, and ancillary 2 proteins, confer protection against GAS challenge in a mouse model.

Methods

We evaluated pilus distribution and conservation by sequencing the subunits of 39 new GAS isolates and used immunoblot analysis and agglutination assays to define the specificity of T sera to pilus subunits.

Results

GAS pili are encoded by 9 different islands within which backbone protein, ancillary protein 1, and ancillary protein 2 cluster in 15, 16, and 5 variants, respectively. Immunoblot and agglutination assays revealed that T type is determined by the backbone variant. This observation enabled us to set up a simple polymerase chain reaction assay to define the T type of GAS isolates.

Conclusions

We propose the use of a tee gene sequence typing, analogous to the emm gene typing, as a valuable molecular tool that could substitute for the serological T classification of GAS strains. From our sequence analysis and from recent epidemiological data, we estimate that a vaccine comprising a combination of 12 backbone variants would protect against > 90% of currently circulating strains."xsd:string
http://purl.uniprot.org/citations/18928376http://purl.org/dc/terms/identifier"doi:10.1086/593176"xsd:string
http://purl.uniprot.org/citations/18928376http://purl.org/dc/terms/identifier"doi:10.1086/593176"xsd:string
http://purl.uniprot.org/citations/18928376http://purl.uniprot.org/core/author"Grandi G."xsd:string
http://purl.uniprot.org/citations/18928376http://purl.uniprot.org/core/author"Grandi G."xsd:string
http://purl.uniprot.org/citations/18928376http://purl.uniprot.org/core/author"Musser J.M."xsd:string
http://purl.uniprot.org/citations/18928376http://purl.uniprot.org/core/author"Musser J.M."xsd:string
http://purl.uniprot.org/citations/18928376http://purl.uniprot.org/core/author"Mora M."xsd:string
http://purl.uniprot.org/citations/18928376http://purl.uniprot.org/core/author"Mora M."xsd:string
http://purl.uniprot.org/citations/18928376http://purl.uniprot.org/core/author"Margarit I."xsd:string
http://purl.uniprot.org/citations/18928376http://purl.uniprot.org/core/author"Margarit I."xsd:string
http://purl.uniprot.org/citations/18928376http://purl.uniprot.org/core/author"Mariani M."xsd:string
http://purl.uniprot.org/citations/18928376http://purl.uniprot.org/core/author"Mariani M."xsd:string
http://purl.uniprot.org/citations/18928376http://purl.uniprot.org/core/author"Telford J.L."xsd:string
http://purl.uniprot.org/citations/18928376http://purl.uniprot.org/core/author"Telford J.L."xsd:string
http://purl.uniprot.org/citations/18928376http://purl.uniprot.org/core/author"Orefici G."xsd:string
http://purl.uniprot.org/citations/18928376http://purl.uniprot.org/core/author"Orefici G."xsd:string
http://purl.uniprot.org/citations/18928376http://purl.uniprot.org/core/author"Falugi F."xsd:string
http://purl.uniprot.org/citations/18928376http://purl.uniprot.org/core/author"Falugi F."xsd:string
http://purl.uniprot.org/citations/18928376http://purl.uniprot.org/core/author"Capo S."xsd:string
http://purl.uniprot.org/citations/18928376http://purl.uniprot.org/core/author"Capo S."xsd:string
http://purl.uniprot.org/citations/18928376http://purl.uniprot.org/core/author"Manetti A.G."xsd:string
http://purl.uniprot.org/citations/18928376http://purl.uniprot.org/core/author"Manetti A.G."xsd:string
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