| http://purl.uniprot.org/citations/18952102 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18952102 | http://www.w3.org/2000/01/rdf-schema#comment | "Long-range cis elements are critical regulators of transcription, particularly for clustered paralogous genes. Such are the five PADI genes in 1p35-36 encoding peptidylarginine deiminases, which catalyze deimination, a Ca2+-dependent post-translational modification. Deimination has been implicated in the pathophysiology of severe human diseases such as multiple sclerosis and rheumatoid arthritis. The PADI genes present different expression patterns. PADI1-3 are expressed in the epidermis, with increased expression levels in the most differentiated keratinocytes. Previous studies on PADI proximal promoters failed to explain such specificity of expression. We identified a conserved intergenic sequence in the PADI locus (IG1), which may play a role in PADI transcriptional regulation. In this work, we identified two DNase I.hypersensitive sites located in IG1, PAD intergenic enhancer segment 1 (PIE-S1) and PIE-S2, which act in synergy as a bipartite enhancer of the PADI3 and probably PADI1 promoters in normal human epidermal keratinocytes differentiated by a high-calcium-containing medium (1.5 mM). PIE-S1 and PIE-S2 present all the hallmarks of transcriptional enhancers: orientation-independence, copy-number dependence and cell-type specificity. PIE-S1 and PIE-S2 comprise conserved putative binding sites for MIBP1/RFX1 and activator protein 1, respectively. Deletion mutant screening revealed that these sites are crucial for the enhancer activity. Furthermore, chromatin immunoprecipitation assays evidenced differential binding of JunD or c-Jun on the activator protein 1 site depending on the cell differentiation state. Our results reveal the molecular bases of the expression specificity of PADI1 and PADI3 during keratinocyte differentiation through a long-range enhancer and support a model of PADI gene regulation depending on c-Jun-JunD competition."xsd:string |
| http://purl.uniprot.org/citations/18952102 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.jmb.2008.10.019"xsd:string |
| http://purl.uniprot.org/citations/18952102 | http://purl.uniprot.org/core/author | "Dong S."xsd:string |
| http://purl.uniprot.org/citations/18952102 | http://purl.uniprot.org/core/author | "Simon M."xsd:string |
| http://purl.uniprot.org/citations/18952102 | http://purl.uniprot.org/core/author | "Ying S."xsd:string |
| http://purl.uniprot.org/citations/18952102 | http://purl.uniprot.org/core/author | "Serre G."xsd:string |
| http://purl.uniprot.org/citations/18952102 | http://purl.uniprot.org/core/author | "Takahara H."xsd:string |
| http://purl.uniprot.org/citations/18952102 | http://purl.uniprot.org/core/author | "Mechin M.C."xsd:string |
| http://purl.uniprot.org/citations/18952102 | http://purl.uniprot.org/core/author | "Chavanas S."xsd:string |
| http://purl.uniprot.org/citations/18952102 | http://purl.uniprot.org/core/author | "Adoue V."xsd:string |
| http://purl.uniprot.org/citations/18952102 | http://purl.uniprot.org/core/author | "Charveron M."xsd:string |
| http://purl.uniprot.org/citations/18952102 | http://purl.uniprot.org/core/author | "Caubet C."xsd:string |
| http://purl.uniprot.org/citations/18952102 | http://purl.uniprot.org/core/author | "Coudane F."xsd:string |
| http://purl.uniprot.org/citations/18952102 | http://purl.uniprot.org/core/author | "Duplan H."xsd:string |
| http://purl.uniprot.org/citations/18952102 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18952102 | http://purl.uniprot.org/core/name | "J Mol Biol"xsd:string |
| http://purl.uniprot.org/citations/18952102 | http://purl.uniprot.org/core/pages | "1048-1057"xsd:string |
| http://purl.uniprot.org/citations/18952102 | http://purl.uniprot.org/core/title | "Long-range enhancer differentially regulated by c-Jun and JunD controls peptidylarginine deiminase-3 gene in keratinocytes."xsd:string |
| http://purl.uniprot.org/citations/18952102 | http://purl.uniprot.org/core/volume | "384"xsd:string |
| http://purl.uniprot.org/citations/18952102 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18952102 |
| http://purl.uniprot.org/citations/18952102 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/18952102 |
| http://purl.uniprot.org/uniprot/#_A0A510GAI3-mappedCitation-18952102 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18952102 |
| http://purl.uniprot.org/uniprot/#_P05412-mappedCitation-18952102 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18952102 |
| http://purl.uniprot.org/uniprot/#_P17535-mappedCitation-18952102 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18952102 |