| http://purl.uniprot.org/citations/18952718 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18952718 | http://www.w3.org/2000/01/rdf-schema#comment | "Hypertension is a major risk factor for stroke, but the factors that contribute to the increased incidence and severity of ischemic stroke in hypertension remain to be determined. 20-hydroxyeicosatetraenoic acid (20-HETE) has been reported to be a potent constrictor of cerebral arteries, and inhibitors of 20-HETE formation reduce infarct size following cerebral ischemia. The present study examined whether elevated production of 20-HETE in the cerebral vasculature could contribute to the larger infarct size previously reported after transient middle cerebral artery occlusion (MCAO) in hypertensive strains of rat [spontaneously hypertensive rat (SHR) and spontaneously hypertensive stroke-prone rat (SHRSP)]. The synthesis of 20-HETE in the cerebral vasculature of SHRSP measured by liquid chromatography-tandem mass spectrometry was about twice that seen in Wistar-Kyoto (WKY) rats. This was associated with the elevated expression of cytochrome P-450 (CYP)4A protein and CYP4A1 and CYP4A8 mRNA. Infarct volume after transient MCAO was greater in SHRSP (36+/-4% of hemisphere volume) than in SHR (19+/-5%) or WKY rats (5+/-2%). This was associated with a significantly greater reduction in regional cerebral blood flow (rCBF) in SHR and SHRSP than in WKY rats during the ischemic period (78% vs. 62%). In WKY rats, rCBF returned to 75% of control following reperfusion. In contrast, SHR and SHRSP exhibited a large (166+/-18% of baseline) and sustained (1 h) postischemic hyperperfusion. Acute blockade of the synthesis of 20-HETE with N-hydroxy-N'-(4-butyl-2-methylphenyl)-formamidine (HET0016; 1 mg/kg) reduced infarct size by 59% in SHR and 87% in SHRSP. HET0016 had no effect on the fall in rCBF during MCAO but eliminated the hyperemic response. HET0016 also attenuated vascular O2*-formation and restored endothelium-dependent dilation in cerebral arteries of SHRSP. These results indicate the production of 20-HETE is elevated in the cerebral vasculature of SHRSP and contributes to oxidative stress, endothelial dysfunction, and the enhanced sensitivity to ischemic stroke in this hypertensive model."xsd:string |
| http://purl.uniprot.org/citations/18952718 | http://purl.org/dc/terms/identifier | "doi:10.1152/ajpheart.00512.2008"xsd:string |
| http://purl.uniprot.org/citations/18952718 | http://purl.uniprot.org/core/author | "Roman R.J."xsd:string |
| http://purl.uniprot.org/citations/18952718 | http://purl.uniprot.org/core/author | "Falck J."xsd:string |
| http://purl.uniprot.org/citations/18952718 | http://purl.uniprot.org/core/author | "Harder D.R."xsd:string |
| http://purl.uniprot.org/citations/18952718 | http://purl.uniprot.org/core/author | "Flasch A.K."xsd:string |
| http://purl.uniprot.org/citations/18952718 | http://purl.uniprot.org/core/author | "Dunn K.M."xsd:string |
| http://purl.uniprot.org/citations/18952718 | http://purl.uniprot.org/core/author | "Renic M."xsd:string |
| http://purl.uniprot.org/citations/18952718 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18952718 | http://purl.uniprot.org/core/name | "Am J Physiol Heart Circ Physiol"xsd:string |
| http://purl.uniprot.org/citations/18952718 | http://purl.uniprot.org/core/pages | "H2455-65"xsd:string |
| http://purl.uniprot.org/citations/18952718 | http://purl.uniprot.org/core/title | "Elevated production of 20-HETE in the cerebral vasculature contributes to severity of ischemic stroke and oxidative stress in spontaneously hypertensive rats."xsd:string |
| http://purl.uniprot.org/citations/18952718 | http://purl.uniprot.org/core/volume | "295"xsd:string |
| http://purl.uniprot.org/citations/18952718 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18952718 |
| http://purl.uniprot.org/citations/18952718 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/18952718 |
| http://purl.uniprot.org/uniprot/#_P08516-mappedCitation-18952718 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18952718 |
| http://purl.uniprot.org/uniprot/#_P24464-mappedCitation-18952718 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18952718 |
| http://purl.uniprot.org/uniprot/P08516 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/18952718 |
| http://purl.uniprot.org/uniprot/P24464 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/18952718 |