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http://purl.uniprot.org/citations/18974390http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/18974390http://www.w3.org/2000/01/rdf-schema#comment"

Purpose

Colon antigen-1 (COA-1) was recently identified as a novel antigen of colorectal cancer encoded by the UBXD5 gene. Here, we evaluated whether a specific T-cell-mediated response directed against this molecule can occur in colorectal cancer patients.

Experimental design

Antigen- and tumor-specific immunologic responses of peripheral blood mononuclear cells (PBMC) stimulated in vitro with the MHC class II-associated immunogenic epitope of COA-1 (FSTFPPTLYQDDTLTLQAAG) were analyzed by IFN-gamma ELISPOT assay.

Results

COA-1-specific and tumor-reactive T lymphocytes were isolated from all (n = 7) HLA-DRbeta1*0402+ or *1301+ colorectal cancer patients with progressive disease (Dukes' C and D) but not in patients (n = 4) with early-stage tumor (Dukes' A and B) and in healthy donors (n = 5), suggesting that the immune response against this antigen is associated with the progression of colorectal cancer. COA-1- and tumor-specific T lymphocytes displayed a CD3+CD4+CD69+CD45RA+ phenotype, compatible with the activated effector-type T-cell subset, and most of them exerted cytotoxic activity against HLA-matched and COA-1+ tumor cells. COA-1-specific T cells could also be isolated by in vitro stimulation of peripheral blood mononuclear cells with autologous dendritic cells loaded with tumor lysate, suggesting that this antigen can generate a dominant immunologic response against colorectal cancer cells. Notably, we could identify also COA-1-derived epitopes binding to HLA-A*0201 molecules that elicited antigen- and tumor-specific CD8+ T-cell-mediated responses in colorectal cancer patients.

Conclusions

Both CD4+ and CD8+ T-cell responses against COA-1 can occur in colorectal cancer patients with metastatic disease, suggesting that this antigen is suitable for immunotherapeutic protocols of these patients."xsd:string
http://purl.uniprot.org/citations/18974390http://purl.org/dc/terms/identifier"doi:10.1158/1078-0432.ccr-08-0832"xsd:string
http://purl.uniprot.org/citations/18974390http://purl.uniprot.org/core/author"Tartaglia M."xsd:string
http://purl.uniprot.org/citations/18974390http://purl.uniprot.org/core/author"Petrangeli V."xsd:string
http://purl.uniprot.org/citations/18974390http://purl.uniprot.org/core/author"Guida S."xsd:string
http://purl.uniprot.org/citations/18974390http://purl.uniprot.org/core/author"Fodale V."xsd:string
http://purl.uniprot.org/citations/18974390http://purl.uniprot.org/core/author"Belardelli F."xsd:string
http://purl.uniprot.org/citations/18974390http://purl.uniprot.org/core/author"Castelli C."xsd:string
http://purl.uniprot.org/citations/18974390http://purl.uniprot.org/core/author"Parmiani G."xsd:string
http://purl.uniprot.org/citations/18974390http://purl.uniprot.org/core/author"Rivoltini L."xsd:string
http://purl.uniprot.org/citations/18974390http://purl.uniprot.org/core/author"D'Agostino G."xsd:string
http://purl.uniprot.org/citations/18974390http://purl.uniprot.org/core/author"Maccalli C."xsd:string
http://purl.uniprot.org/citations/18974390http://purl.uniprot.org/core/author"Di Cristanziano V."xsd:string
http://purl.uniprot.org/citations/18974390http://purl.uniprot.org/core/author"Mazzocchi A."xsd:string
http://purl.uniprot.org/citations/18974390http://purl.uniprot.org/core/author"Perrone M.P."xsd:string
http://purl.uniprot.org/citations/18974390http://purl.uniprot.org/core/author"Laurenti L."xsd:string
http://purl.uniprot.org/citations/18974390http://purl.uniprot.org/core/author"Arienti F."xsd:string
http://purl.uniprot.org/citations/18974390http://purl.uniprot.org/core/author"Corsi D."xsd:string
http://purl.uniprot.org/citations/18974390http://purl.uniprot.org/core/author"Zagonel V."xsd:string
http://purl.uniprot.org/citations/18974390http://purl.uniprot.org/core/date"2008"xsd:gYear
http://purl.uniprot.org/citations/18974390http://purl.uniprot.org/core/name"Clin Cancer Res"xsd:string
http://purl.uniprot.org/citations/18974390http://purl.uniprot.org/core/pages"7292-7303"xsd:string
http://purl.uniprot.org/citations/18974390http://purl.uniprot.org/core/title"Induction of both CD8+ and CD4+ T-cell-mediated responses in colorectal cancer patients by colon antigen-1."xsd:string
http://purl.uniprot.org/citations/18974390http://purl.uniprot.org/core/volume"14"xsd:string
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