| http://purl.uniprot.org/citations/18981107 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18981107 | http://www.w3.org/2000/01/rdf-schema#comment | "HIV-1 envelope (Env) triggers membrane fusion between the virus and the target cell. The cellular mechanism underlying this process is not well known. Phosphatidylinositol 4,5-bisphosphate (PIP(2)) is known to be important for the late steps of the HIV-1 infection cycle by promoting Gag localization to the plasma membrane during viral assembly, but it has not been implicated in early stages of HIV-1 membrane-related events. In this study, we show that binding of the initial HIV-1 Env-gp120 protein induces PIP(2) production in permissive lymphocytes through the activation of phosphatidylinositol-4-phosphate 5-kinase (PI4P5-K) Ialpha. Overexpression of wild-type PI4P5-K Ialpha increased HIV-1 Env-mediated PIP(2) production and enhanced viral replication in primary lymphocytes and CEM T cells, whereas PIP(2) production and HIV-1 infection were both severely reduced in cells overexpressing the kinase-dead mutant D227A (D/A)-PI4P5-K Ialpha. Similar results were obtained with replicative and single-cycle HIV-1 particles. HIV-1 infection was also inhibited by knockdown of endogenous expression of PI4P5-K Ialpha. These data indicate that PI4P5-K Ialpha-mediated PIP(2) production is crucial for HIV-1 entry and the early steps of infection in permissive lymphocytes."xsd:string |
| http://purl.uniprot.org/citations/18981107 | http://purl.org/dc/terms/identifier | "doi:10.4049/jimmunol.181.10.6882"xsd:string |
| http://purl.uniprot.org/citations/18981107 | http://purl.uniprot.org/core/author | "Sanchez-Madrid F."xsd:string |
| http://purl.uniprot.org/citations/18981107 | http://purl.uniprot.org/core/author | "Munoz-Fernandez M.A."xsd:string |
| http://purl.uniprot.org/citations/18981107 | http://purl.uniprot.org/core/author | "Barrero-Villar M."xsd:string |
| http://purl.uniprot.org/citations/18981107 | http://purl.uniprot.org/core/author | "Cabrero J.R."xsd:string |
| http://purl.uniprot.org/citations/18981107 | http://purl.uniprot.org/core/author | "Gordon-Alonso M."xsd:string |
| http://purl.uniprot.org/citations/18981107 | http://purl.uniprot.org/core/author | "Barroso-Gonzalez J."xsd:string |
| http://purl.uniprot.org/citations/18981107 | http://purl.uniprot.org/core/author | "Valenzuela-Fernandez A."xsd:string |
| http://purl.uniprot.org/citations/18981107 | http://purl.uniprot.org/core/author | "Alvarez-Losada S."xsd:string |
| http://purl.uniprot.org/citations/18981107 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18981107 | http://purl.uniprot.org/core/name | "J Immunol"xsd:string |
| http://purl.uniprot.org/citations/18981107 | http://purl.uniprot.org/core/pages | "6882-6888"xsd:string |
| http://purl.uniprot.org/citations/18981107 | http://purl.uniprot.org/core/title | "PI4P5-kinase Ialpha is required for efficient HIV-1 entry and infection of T cells."xsd:string |
| http://purl.uniprot.org/citations/18981107 | http://purl.uniprot.org/core/volume | "181"xsd:string |
| http://purl.uniprot.org/citations/18981107 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18981107 |
| http://purl.uniprot.org/citations/18981107 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/18981107 |
| http://purl.uniprot.org/uniprot/#_B4DY52-mappedCitation-18981107 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18981107 |
| http://purl.uniprot.org/uniprot/#_A6PW57-mappedCitation-18981107 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18981107 |
| http://purl.uniprot.org/uniprot/#_P78517-mappedCitation-18981107 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18981107 |
| http://purl.uniprot.org/uniprot/#_Q99755-mappedCitation-18981107 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18981107 |
| http://purl.uniprot.org/uniprot/P78517 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/18981107 |
| http://purl.uniprot.org/uniprot/A6PW57 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/18981107 |
| http://purl.uniprot.org/uniprot/Q99755 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/18981107 |