| http://purl.uniprot.org/citations/18981233 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18981233 | http://www.w3.org/2000/01/rdf-schema#comment | "Here we investigate the role of rab5 and its cognate exchange factors rabex-5 and hRME-6 in the regulation of AP2 uncoating from endocytic clathrin-coated vesicles (CCVs). In vitro, we show that the rate of AP2 uncoating from CCVs is dependent on the level of functional rab5. In vivo, overexpression of dominant-negative rab5(S34N), or small interfering RNA (siRNA)-mediated depletion of hRME-6, but not rabex-5, resulted in increased steady-state levels of AP2 associated with endocytic vesicles, which is consistent with reduced uncoating efficiency. hRME-6 guanine nucleotide exchange factor activity requires hRME-6 binding to alpha-adaptin ear, which displaces the ear-associated mu2 kinase AAK1. siRNA-mediated depletion of hRME-6 increases phospho-mu2 levels, and expression of a phosphomimetic mu2 mutant increases levels of endocytic vesicle-associated AP2. Depletion of hRME-6 or rab5(S35N) expression also increases the levels of phosphoinositide 4,5-bisphosphate (PtdIns(4,5)P(2)) associated with endocytic vesicles. These data are consistent with a model in which hRME-6 and rab5 regulate AP2 uncoating in vivo by coordinately regulating mu2 dephosphorylation and PtdIns(4,5)P(2) levels in CCVs."xsd:string |
| http://purl.uniprot.org/citations/18981233 | http://purl.org/dc/terms/identifier | "doi:10.1083/jcb.200806016"xsd:string |
| http://purl.uniprot.org/citations/18981233 | http://purl.uniprot.org/core/author | "Singh S."xsd:string |
| http://purl.uniprot.org/citations/18981233 | http://purl.uniprot.org/core/author | "Schiavo G."xsd:string |
| http://purl.uniprot.org/citations/18981233 | http://purl.uniprot.org/core/author | "Grant B.D."xsd:string |
| http://purl.uniprot.org/citations/18981233 | http://purl.uniprot.org/core/author | "Hansen J."xsd:string |
| http://purl.uniprot.org/citations/18981233 | http://purl.uniprot.org/core/author | "Smythe E."xsd:string |
| http://purl.uniprot.org/citations/18981233 | http://purl.uniprot.org/core/author | "Fonarev P."xsd:string |
| http://purl.uniprot.org/citations/18981233 | http://purl.uniprot.org/core/author | "Maxwell E."xsd:string |
| http://purl.uniprot.org/citations/18981233 | http://purl.uniprot.org/core/author | "Semerdjieva S."xsd:string |
| http://purl.uniprot.org/citations/18981233 | http://purl.uniprot.org/core/author | "Shortt B."xsd:string |
| http://purl.uniprot.org/citations/18981233 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18981233 | http://purl.uniprot.org/core/name | "J Cell Biol"xsd:string |
| http://purl.uniprot.org/citations/18981233 | http://purl.uniprot.org/core/pages | "499-511"xsd:string |
| http://purl.uniprot.org/citations/18981233 | http://purl.uniprot.org/core/title | "Coordinated regulation of AP2 uncoating from clathrin-coated vesicles by rab5 and hRME-6."xsd:string |
| http://purl.uniprot.org/citations/18981233 | http://purl.uniprot.org/core/volume | "183"xsd:string |
| http://purl.uniprot.org/citations/18981233 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18981233 |
| http://purl.uniprot.org/citations/18981233 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/18981233 |
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| http://purl.uniprot.org/uniprot/#_Q14247-mappedCitation-18981233 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18981233 |
| http://purl.uniprot.org/uniprot/#_Q15642-mappedCitation-18981233 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18981233 |
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| http://purl.uniprot.org/uniprot/#_O15145-mappedCitation-18981233 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18981233 |