http://purl.uniprot.org/citations/18983665 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/18983665 | http://www.w3.org/2000/01/rdf-schema#comment | "BackgroundBesides functioning as chemosensors for a broad range of endogenous and synthetic ligands, transient receptor potential vanilloid (TRPV) 1-4 channels have also been related to capsaicin (TRPV1), pain, and thermal stimuli perception, and itching sensation (TRPV1-4). While the expression of the TRPV1-4 genes has been adequately proved in skin, sensory fibres and keratinocytes, less is known about TRPV3 and TRPV4 expression in human blood cells.ResultsTo study the gene expression of TRPV1-4 genes in human leukocytes, a quantitative Real-Time PCR (qRT-PCR) method, based on the calculation of their relative expression, has been developed and validated. The four commonly used house-keeping genes (HKGs), beta-Actin (Act-B), glyceraldehyde-3P-dehydrogenase (GAPDH), hypoxanthine ribosyltransferase (HPRT1), and cyclophilin B (hCyPB), were tested for the stability of their expression in several human leukocyte samples, and used in the normalization procedure to determine the mRNA levels of the TRPV 1-4 genes in 30 healthy subjects. cDNAs belonging to all the TRPV1-4 genes were detected in leukocytes but the genes appear to be expressed at different levels. Our analysis did not show significant sex differences in TRPV1-4 cDNA levels in the 30 healthy subjects. The same qRT-PCR assay was used to compare TRPV1-4 expression between healthy controls and patients hyposensitive to capsaicin, pain and thermal stimuli: an almost doubled up-regulation of the TRPV1 gene was found in the pathological subjects.ConclusionThe qRT-PCR assay developed and tested in this study allowed us to determine the relative expression of TRPV1-4 genes in human leukocytes: TRPV3 is the least expressed gene of this pool, followed by TRPV4, TRPV1 and TRPV2. The comparison of TRPV1-4 gene expression between two groups of healthy and hyposensitive subjects highlighted the evident up-regulation of TRPV1, which was almost doubly expressed (1.9x normalized fold induction) in the latter group. All the four house-keeping genes tested in this work (Act-B, GAPDH, hCyPB, HPRT1) were classified as optimal controls and showed a constant expression in human leukocytes samples. We recommend the use of these genes in similar qRT-PCR studies on human blood cells."xsd:string |
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http://purl.uniprot.org/citations/18983665 | http://purl.uniprot.org/core/author | "Frati F."xsd:string |
http://purl.uniprot.org/citations/18983665 | http://purl.uniprot.org/core/author | "Aloisi A.M."xsd:string |
http://purl.uniprot.org/citations/18983665 | http://purl.uniprot.org/core/author | "Bachiocco V."xsd:string |
http://purl.uniprot.org/citations/18983665 | http://purl.uniprot.org/core/author | "Marsili L."xsd:string |
http://purl.uniprot.org/citations/18983665 | http://purl.uniprot.org/core/author | "Spinsanti G."xsd:string |
http://purl.uniprot.org/citations/18983665 | http://purl.uniprot.org/core/author | "Panti C."xsd:string |
http://purl.uniprot.org/citations/18983665 | http://purl.uniprot.org/core/author | "Ceccarelli I."xsd:string |
http://purl.uniprot.org/citations/18983665 | http://purl.uniprot.org/core/author | "Zannolli R."xsd:string |
http://purl.uniprot.org/citations/18983665 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
http://purl.uniprot.org/citations/18983665 | http://purl.uniprot.org/core/name | "Mol Pain"xsd:string |
http://purl.uniprot.org/citations/18983665 | http://purl.uniprot.org/core/pages | "51"xsd:string |
http://purl.uniprot.org/citations/18983665 | http://purl.uniprot.org/core/title | "Quantitative Real-Time PCR detection of TRPV1-4 gene expression in human leukocytes from healthy and hyposensitive subjects."xsd:string |
http://purl.uniprot.org/citations/18983665 | http://purl.uniprot.org/core/volume | "4"xsd:string |
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