| http://purl.uniprot.org/citations/18983992 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18983992 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18983992 | http://www.w3.org/2000/01/rdf-schema#comment | "Functional genomics data suggests that the metabolism of mannitol in the human pathogen Aspergillus fumigatus involves the action of two polyol-specific long-chain dehydrogenases/reductases, mannitol-1-phosphate 5-dehydrogenase (M1PDH) and mannitol 2-dehydrogenase (M2DH). The gene encoding the putative M2DH was expressed in Escherichia coli, and the purified recombinant protein was characterized biochemically. The predicted enzymatic function of a NAD(+)-dependent M2DH was confirmed. The enzyme is a monomer of 58kDa in solution and does not require metals for activity. pH profiles for M2DH and the previously isolated M1PDH were recorded in the pH range 6.0-10.0 for the oxidative and reductive direction of the reactions under conditions where substrate was limiting (k(cat)/K) or saturating (k(cat)). The pH-dependence of logk(cat) was usually different from that of log(k(cat)/K), suggesting that more than one step of the enzymatic mechanism was affected by changes in pH. The greater complexity of the pH profiles of log(k(cat)/K) for the fungal enzymes as compared to the analogous pH profiles for M2DH from Pseudomonas fluorescens may reflect sequence changes in vicinity of the conserved catalytic lysine."xsd:string |
| http://purl.uniprot.org/citations/18983992 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.cbi.2008.10.001"xsd:string |
| http://purl.uniprot.org/citations/18983992 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.cbi.2008.10.001"xsd:string |
| http://purl.uniprot.org/citations/18983992 | http://purl.uniprot.org/core/author | "Nidetzky B."xsd:string |
| http://purl.uniprot.org/citations/18983992 | http://purl.uniprot.org/core/author | "Nidetzky B."xsd:string |
| http://purl.uniprot.org/citations/18983992 | http://purl.uniprot.org/core/author | "Armao G.C."xsd:string |
| http://purl.uniprot.org/citations/18983992 | http://purl.uniprot.org/core/author | "Armao G.C."xsd:string |
| http://purl.uniprot.org/citations/18983992 | http://purl.uniprot.org/core/author | "Bubner P."xsd:string |
| http://purl.uniprot.org/citations/18983992 | http://purl.uniprot.org/core/author | "Bubner P."xsd:string |
| http://purl.uniprot.org/citations/18983992 | http://purl.uniprot.org/core/author | "Klimacek M."xsd:string |
| http://purl.uniprot.org/citations/18983992 | http://purl.uniprot.org/core/author | "Klimacek M."xsd:string |
| http://purl.uniprot.org/citations/18983992 | http://purl.uniprot.org/core/author | "Krahulec S."xsd:string |
| http://purl.uniprot.org/citations/18983992 | http://purl.uniprot.org/core/author | "Krahulec S."xsd:string |
| http://purl.uniprot.org/citations/18983992 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/18983992 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/18983992 | http://purl.uniprot.org/core/name | "Chem. Biol. Interact."xsd:string |
| http://purl.uniprot.org/citations/18983992 | http://purl.uniprot.org/core/name | "Chem. Biol. Interact."xsd:string |
| http://purl.uniprot.org/citations/18983992 | http://purl.uniprot.org/core/pages | "274-282"xsd:string |
| http://purl.uniprot.org/citations/18983992 | http://purl.uniprot.org/core/pages | "274-282"xsd:string |
| http://purl.uniprot.org/citations/18983992 | http://purl.uniprot.org/core/title | "Polyol-specific long-chain dehydrogenases/reductases of mannitol metabolism in Aspergillus fumigatus: biochemical characterization and pH studies of mannitol 2-dehydrogenase and mannitol-1-phosphate 5-dehydrogenase."xsd:string |
| http://purl.uniprot.org/citations/18983992 | http://purl.uniprot.org/core/title | "Polyol-specific long-chain dehydrogenases/reductases of mannitol metabolism in Aspergillus fumigatus: biochemical characterization and pH studies of mannitol 2-dehydrogenase and mannitol-1-phosphate 5-dehydrogenase."xsd:string |
| http://purl.uniprot.org/citations/18983992 | http://purl.uniprot.org/core/volume | "178"xsd:string |
| http://purl.uniprot.org/citations/18983992 | http://purl.uniprot.org/core/volume | "178"xsd:string |