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http://purl.uniprot.org/citations/18987160http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/18987160http://www.w3.org/2000/01/rdf-schema#comment"

Background

Mature spermatozoa contain thousands of mRNA transcripts. It has been recently shown that human sperm can deliver RNA into the oocyte, suggesting that mRNAs might have a role before or after fertilization. Human embryos express PSG1 (pregnancy-specific beta-1-glycoprotein 1) and HLA-E (human leukocyte antigen-E), molecules playing a role in implantation and early development. We compared PSG1 and HLA-E sperm mRNA levels in fertile and infertile men and we tested the hypothesis that these transcripts are selectively retained in the newly formed zygote.

Methods

Real-time RT-PCR was used to analyze sperm mRNA levels (n = 11 fertile, n = 31 infertile patients) of PSG1, HLA-E and PRM2 (protamine 2). The presence of PSG1 and HLA-E proteins was evaluated by western blot in sperm protein extracts (n = 3). Using ICSI of human sperm into hamster oocytes we evaluated the permanence of these mRNAs at different time points (n = 10 for each time) after fertilization.

Results

PSG1, HLA-E and PRM2 transcripts were demonstrated in ejaculated sperm. The fertile group showed significantly higher levels of PSG1 and HLA-E mRNA (both P < 0.05) than the infertile group, whereas PRM2 levels were not significantly different. However, PSG1 and HLA-E proteins were not found in ejaculated sperm. Following ICSI, PRM2 was undetectable after fertilization; conversely, PSG1 and HLA-E transcripts remained detectable for at least 24 h of zygotic development.

Conclusions

We provide new evidence that indicates that human sperm deliver transcripts that may have a role in early embryo development and decreased levels of these transcripts may be associated with infertility."xsd:string
http://purl.uniprot.org/citations/18987160http://purl.org/dc/terms/identifier"doi:10.1093/humrep/den381"xsd:string
http://purl.uniprot.org/citations/18987160http://purl.uniprot.org/core/author"Jones E."xsd:string
http://purl.uniprot.org/citations/18987160http://purl.uniprot.org/core/author"Franchi A."xsd:string
http://purl.uniprot.org/citations/18987160http://purl.uniprot.org/core/author"Oehninger S."xsd:string
http://purl.uniprot.org/citations/18987160http://purl.uniprot.org/core/author"Avendano C."xsd:string
http://purl.uniprot.org/citations/18987160http://purl.uniprot.org/core/date"2009"xsd:gYear
http://purl.uniprot.org/citations/18987160http://purl.uniprot.org/core/name"Hum Reprod"xsd:string
http://purl.uniprot.org/citations/18987160http://purl.uniprot.org/core/pages"270-277"xsd:string
http://purl.uniprot.org/citations/18987160http://purl.uniprot.org/core/title"Pregnancy-specific {beta}-1-glycoprotein 1 and human leukocyte antigen-E mRNA in human sperm: differential expression in fertile and infertile men and evidence of a possible functional role during early development."xsd:string
http://purl.uniprot.org/citations/18987160http://purl.uniprot.org/core/volume"24"xsd:string
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