| http://purl.uniprot.org/citations/19005168 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19005168 | http://www.w3.org/2000/01/rdf-schema#comment | "Epidermal growth factor (EGF) has been shown to stimulate survival in diverse cells in vitro. In the present study, the effects of EGF and the EGF-related signaling pathway on proliferation of chicken primordial germ cells (PGCs) were investigated. Results showed that EGF (10-100 ng/ml) increased the number and area of PGC colonies in a time- and dose-dependent manner. EGF also activated PKC, a process that was inhibited by AG1478 (an EGFR tyrosine kinase inhibitor) and ethyleneglycol-bis-(beta-aminoethyl ether)-N,N'-tetraacetic acid (EGTA; an intracellular Ca(2+) chelator). In addition, the degradation of NFKBIA and NFKB1 (p65) translocation was observed after EGF treatment, which was significantly blocked by pretreatment with AG1478, EGTA, H(7), or SN50 (NFKB1-specific inhibitor). Furthermore, we found that EGF-induced cell proliferation was significantly attenuated by AG1478, EGTA, H(7), and SN50, respectively. On the other hand, inhibition of EGFR, Ca(2+)/PKC, or NFKB1 abolished the EGF-stimulated increase in the expression of cyclins CCND1 and CCNE1, cyclin-dependent kinase 6 (CDK6), CDK2, and BCL2, and restored the EGF-induced inhibition of BAX expression and caspase 3/9 activity, indicating that EGFR, PKC, and NFKB1 signaling cascades were involved in EGF-stimulated DNA synthesis and antiapoptosis action. In conclusion, EGF stimulated proliferation of chicken PGCs via activation of Ca(2+)/PKC involving NFKB1 signaling pathway. These observations suggest that EGF signaling is important in regulating germ cell proliferation in the chicken embryonic gonad."xsd:string |
| http://purl.uniprot.org/citations/19005168 | http://purl.org/dc/terms/identifier | "doi:10.1095/biolreprod.108.072728"xsd:string |
| http://purl.uniprot.org/citations/19005168 | http://purl.uniprot.org/core/author | "Zhang C."xsd:string |
| http://purl.uniprot.org/citations/19005168 | http://purl.uniprot.org/core/author | "Yu M."xsd:string |
| http://purl.uniprot.org/citations/19005168 | http://purl.uniprot.org/core/author | "Ge C."xsd:string |
| http://purl.uniprot.org/citations/19005168 | http://purl.uniprot.org/core/author | "Petitte J.N."xsd:string |
| http://purl.uniprot.org/citations/19005168 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19005168 | http://purl.uniprot.org/core/name | "Biol Reprod"xsd:string |
| http://purl.uniprot.org/citations/19005168 | http://purl.uniprot.org/core/pages | "528-536"xsd:string |
| http://purl.uniprot.org/citations/19005168 | http://purl.uniprot.org/core/title | "Epidermal growth factor-induced proliferation of chicken primordial germ cells: involvement of calcium/protein kinase C and NFKB1."xsd:string |
| http://purl.uniprot.org/citations/19005168 | http://purl.uniprot.org/core/volume | "80"xsd:string |
| http://purl.uniprot.org/citations/19005168 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19005168 |
| http://purl.uniprot.org/citations/19005168 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19005168 |
| http://purl.uniprot.org/uniprot/#_Q04861-mappedCitation-19005168 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19005168 |
| http://purl.uniprot.org/uniprot/#_Q6PPB4-mappedCitation-19005168 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19005168 |
| http://purl.uniprot.org/uniprot/Q04861 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/19005168 |
| http://purl.uniprot.org/uniprot/Q6PPB4 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/19005168 |