| http://purl.uniprot.org/citations/19008859 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19008859 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19008859 | http://www.w3.org/2000/01/rdf-schema#comment | "Serum response factor transcriptional activity is controlled through interactions with regulatory cofactors such as the coactivator MAL/MRTF-A (myocardin-related transcription factor A). MAL is itself regulated in vivo by changes in cellular actin dynamics, which alter its interaction with G-actin. The G-actin-sensing mechanism of MAL/MRTF-A resides in its N-terminal domain, which consists of three tandem RPEL repeats. We describe the first molecular insights into RPEL function obtained from structures of two independent RPEL(MAL) peptide:G-actin complexes. Both RPEL peptides bind to the G-actin hydrophobic cleft and to subdomain 3. These RPEL(MAL):G-actin structures explain the sequence conservation defining the RPEL motif, including the invariant arginine. Characterisation of the RPEL(MAL):G-actin interaction by fluorescence anisotropy and cell reporter-based assays validates the significance of actin-binding residues for proper MAL localisation and regulation in vivo. We identify important differences in G-actin engagement between the two RPEL(MAL) structures. Comparison with other actin-binding proteins reveals an unexpected similarity to the vitamin-D-binding protein, extending the G-actin-binding protein repertoire."xsd:string |
| http://purl.uniprot.org/citations/19008859 | http://purl.org/dc/terms/identifier | "doi:10.1038/emboj.2008.235"xsd:string |
| http://purl.uniprot.org/citations/19008859 | http://purl.org/dc/terms/identifier | "doi:10.1038/emboj.2008.235"xsd:string |
| http://purl.uniprot.org/citations/19008859 | http://purl.uniprot.org/core/author | "McDonald N.Q."xsd:string |
| http://purl.uniprot.org/citations/19008859 | http://purl.uniprot.org/core/author | "McDonald N.Q."xsd:string |
| http://purl.uniprot.org/citations/19008859 | http://purl.uniprot.org/core/author | "Mouilleron S."xsd:string |
| http://purl.uniprot.org/citations/19008859 | http://purl.uniprot.org/core/author | "Mouilleron S."xsd:string |
| http://purl.uniprot.org/citations/19008859 | http://purl.uniprot.org/core/author | "Treisman R."xsd:string |
| http://purl.uniprot.org/citations/19008859 | http://purl.uniprot.org/core/author | "Treisman R."xsd:string |
| http://purl.uniprot.org/citations/19008859 | http://purl.uniprot.org/core/author | "Guettler S."xsd:string |
| http://purl.uniprot.org/citations/19008859 | http://purl.uniprot.org/core/author | "Guettler S."xsd:string |
| http://purl.uniprot.org/citations/19008859 | http://purl.uniprot.org/core/author | "Langer C.A."xsd:string |
| http://purl.uniprot.org/citations/19008859 | http://purl.uniprot.org/core/author | "Langer C.A."xsd:string |
| http://purl.uniprot.org/citations/19008859 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/19008859 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/19008859 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/19008859 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/19008859 | http://purl.uniprot.org/core/pages | "3198-3208"xsd:string |
| http://purl.uniprot.org/citations/19008859 | http://purl.uniprot.org/core/pages | "3198-3208"xsd:string |
| http://purl.uniprot.org/citations/19008859 | http://purl.uniprot.org/core/title | "Molecular basis for G-actin binding to RPEL motifs from the serum response factor coactivator MAL."xsd:string |
| http://purl.uniprot.org/citations/19008859 | http://purl.uniprot.org/core/title | "Molecular basis for G-actin binding to RPEL motifs from the serum response factor coactivator MAL."xsd:string |
| http://purl.uniprot.org/citations/19008859 | http://purl.uniprot.org/core/volume | "27"xsd:string |
| http://purl.uniprot.org/citations/19008859 | http://purl.uniprot.org/core/volume | "27"xsd:string |