| http://purl.uniprot.org/citations/19013176 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19013176 | http://www.w3.org/2000/01/rdf-schema#comment | "Protein import into mitochondria requires unfolding of the folded mature domain of precursor proteins. Here we compared the effects of amino-acid replacement between the core region and the N-terminal region of the titin I27 domain (the 27th Ig domain of human titin) on its import into isolated mitochondria when attached to a short presequence (pb(2)(35)). We found that several mutations in the core region around Trp34 of the I27 domain enhanced the import rates of the fusion proteins, while the N-terminal K6P mutation, which increases mechanical stability around the N-terminal region, decreases the import rate. When the K6P mutation is combined with core-destabilizing mutations, the import rates of the fusion proteins still decrease, unless a long segment is deleted. These results suggest that mutations in the core region could destabilize the transition state for unfolding from the intermediate with the detached N-terminal segment during import, leading to enhanced unfolding rates, although stabilization of the N-terminal region masks these effects. In other words, the rate-limiting step of the global unfolding upon import into mitochondria switches, depending on the balance between the stability of the N-terminal structure and the stability of the core region of the I27 domain."xsd:string |
| http://purl.uniprot.org/citations/19013176 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.jmb.2008.10.076"xsd:string |
| http://purl.uniprot.org/citations/19013176 | http://purl.uniprot.org/core/author | "Endo T."xsd:string |
| http://purl.uniprot.org/citations/19013176 | http://purl.uniprot.org/core/author | "Sato T."xsd:string |
| http://purl.uniprot.org/citations/19013176 | http://purl.uniprot.org/core/author | "Oguro T."xsd:string |
| http://purl.uniprot.org/citations/19013176 | http://purl.uniprot.org/core/author | "Yamano K."xsd:string |
| http://purl.uniprot.org/citations/19013176 | http://purl.uniprot.org/core/author | "Momose T."xsd:string |
| http://purl.uniprot.org/citations/19013176 | http://purl.uniprot.org/core/author | "Yagawa K."xsd:string |
| http://purl.uniprot.org/citations/19013176 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19013176 | http://purl.uniprot.org/core/name | "J Mol Biol"xsd:string |
| http://purl.uniprot.org/citations/19013176 | http://purl.uniprot.org/core/pages | "811-819"xsd:string |
| http://purl.uniprot.org/citations/19013176 | http://purl.uniprot.org/core/title | "Structural stabilities of different regions of the titin I27 domain contribute differently to unfolding upon mitochondrial protein import."xsd:string |
| http://purl.uniprot.org/citations/19013176 | http://purl.uniprot.org/core/volume | "385"xsd:string |
| http://purl.uniprot.org/citations/19013176 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19013176 |
| http://purl.uniprot.org/citations/19013176 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19013176 |
| http://purl.uniprot.org/uniprot/#_A0A0A0MRA3-mappedCitation-19013176 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19013176 |
| http://purl.uniprot.org/uniprot/#_A0A0A0MTS7-mappedCitation-19013176 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19013176 |
| http://purl.uniprot.org/uniprot/#_A0A0C4DG59-mappedCitation-19013176 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19013176 |
| http://purl.uniprot.org/uniprot/#_A2TKE3-mappedCitation-19013176 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19013176 |
| http://purl.uniprot.org/uniprot/#_A2TKE4-mappedCitation-19013176 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19013176 |
| http://purl.uniprot.org/uniprot/#_A2TKE5-mappedCitation-19013176 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19013176 |
| http://purl.uniprot.org/uniprot/#_A2TKE6-mappedCitation-19013176 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19013176 |
| http://purl.uniprot.org/uniprot/#_B1A7R7-mappedCitation-19013176 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19013176 |
| http://purl.uniprot.org/uniprot/#_C0JYZ2-mappedCitation-19013176 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19013176 |