| http://purl.uniprot.org/citations/19016306 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19016306 | http://www.w3.org/2000/01/rdf-schema#comment | "Human transcription co-regulator SNW1/SKIP is implicated in the regulation of both transcription elongation and alternative splicing. Prp45, the SNW/SKIP ortholog in yeast, is assumed to be essential for pre-mRNA processing. Here, we characterize prp45(1-169), a temperature sensitive allele of PRP45, which at permissive temperature elicits cell division defects and hypersensitivity to microtubule inhibitors. Using a synthetic lethality screen, we found that prp45(1-169) genetically interacts with alleles of NTC members SYF1, CLF1/SYF3, NTC20, and CEF1, and 2nd step splicing factors SLU7, PRP17, PRP18, and PRP22. Cwc2-associated spliceosomal complexes purified from prp45(1-169) cells showed decreased stoichiometry of Prp22, suggesting its deranged interaction with the spliceosome. In vivo splicing assays in prp45(1-169) cells revealed that branch point mutants accumulated more pre-mRNA whereas 5' and 3' splice site mutants showed elevated levels of lariat-exon intermediate as compared to wild-type cells. Splicing of canonical intron was unimpeded. Notably, the expression of Prp45(119-379) in prp45(1-169) cells restored Prp22 partition in the Cwc2-pulldowns and rescued temperature sensitivity and splicing phenotype of prp45(1-169) strain. Our data suggest that Prp45 contributes, in part through its interaction with the 2nd step-proofreading helicase Prp22, to splicing efficiency of substrates non-conforming to the consensus."xsd:string |
| http://purl.uniprot.org/citations/19016306 | http://purl.org/dc/terms/identifier | "doi:10.1002/jcb.21989"xsd:string |
| http://purl.uniprot.org/citations/19016306 | http://purl.uniprot.org/core/author | "Folk P."xsd:string |
| http://purl.uniprot.org/citations/19016306 | http://purl.uniprot.org/core/author | "Puta F."xsd:string |
| http://purl.uniprot.org/citations/19016306 | http://purl.uniprot.org/core/author | "Skruzny M."xsd:string |
| http://purl.uniprot.org/citations/19016306 | http://purl.uniprot.org/core/author | "Gahura O."xsd:string |
| http://purl.uniprot.org/citations/19016306 | http://purl.uniprot.org/core/author | "Munzarova V."xsd:string |
| http://purl.uniprot.org/citations/19016306 | http://purl.uniprot.org/core/author | "Abrhamova K."xsd:string |
| http://purl.uniprot.org/citations/19016306 | http://purl.uniprot.org/core/author | "Valentova A."xsd:string |
| http://purl.uniprot.org/citations/19016306 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19016306 | http://purl.uniprot.org/core/name | "J Cell Biochem"xsd:string |
| http://purl.uniprot.org/citations/19016306 | http://purl.uniprot.org/core/pages | "139-151"xsd:string |
| http://purl.uniprot.org/citations/19016306 | http://purl.uniprot.org/core/title | "Prp45 affects Prp22 partition in spliceosomal complexes and splicing efficiency of non-consensus substrates."xsd:string |
| http://purl.uniprot.org/citations/19016306 | http://purl.uniprot.org/core/volume | "106"xsd:string |
| http://purl.uniprot.org/citations/19016306 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19016306 |
| http://purl.uniprot.org/citations/19016306 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19016306 |
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| http://purl.uniprot.org/uniprot/#_Q12309-mappedCitation-19016306 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19016306 |
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