| http://purl.uniprot.org/citations/19017395 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19017395 | http://www.w3.org/2000/01/rdf-schema#comment | "BackgroundThe understanding of cutaneous pigmentation biology is relevant from the biologic and clinical point of view. The binding of alpha-melanocortin and its specific receptor, on the plasma membrane of melanin synthesising cells, plays a crucial role in melanins biosynthesis. Furthermore, loss of MC1R function is associated with an increased incidence of melanoma and non-melanoma skin cancer. The expression of the alpha-melanocortin receptor gene is highly controlled but, at the present, region responsible for tissue-specific activity of the gene promoter has not been identified.MethodsWe have cloned the genomic sequences upstream the human MC1R coding gene. A DNA fragment of 5 kilobases upstream the human MC1R encoding sequence was placed in front of a reporter gene and several deletion mutants of such fragment have been prepared. These constructs have been tested for the ability to drive the melanocyte-specific gene expression of the reporter gene using transfection experiments in melanocyte and non-melanocyte cell lines. From these experiments we identified a DNA fragment with the ability to drive the gene transcription in a tissue-specific way and we used this small DNA fragment in DNA-protein interaction assays.ResultsWe show that the 150 base pairs upstream the MC1R gene initiation codon are able to drive the melanocyte-specific gene transcription. Furthermore, we provide experimental evidences suggesting that on such minimal melanocyte-specific gene promoter can assemble tissue-specific complexes.ConclusionThe present results strongly imply that the transcriptional regulation of the melanocyte-specific MC1R gene requires an internal promoter located in the 150 base pairs upstream the initiation codon."xsd:string |
| http://purl.uniprot.org/citations/19017395 | http://purl.org/dc/terms/identifier | "doi:10.1186/1756-9966-27-71"xsd:string |
| http://purl.uniprot.org/citations/19017395 | http://purl.uniprot.org/core/author | "Picardo M."xsd:string |
| http://purl.uniprot.org/citations/19017395 | http://purl.uniprot.org/core/author | "Natali P.G."xsd:string |
| http://purl.uniprot.org/citations/19017395 | http://purl.uniprot.org/core/author | "Pascucci B."xsd:string |
| http://purl.uniprot.org/citations/19017395 | http://purl.uniprot.org/core/author | "Civitareale D."xsd:string |
| http://purl.uniprot.org/citations/19017395 | http://purl.uniprot.org/core/author | "Miccadei S."xsd:string |
| http://purl.uniprot.org/citations/19017395 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/19017395 | http://purl.uniprot.org/core/name | "J Exp Clin Cancer Res"xsd:string |
| http://purl.uniprot.org/citations/19017395 | http://purl.uniprot.org/core/pages | "71"xsd:string |
| http://purl.uniprot.org/citations/19017395 | http://purl.uniprot.org/core/title | "Identification of the minimal melanocyte-specific promoter in the melanocortin receptor 1 gene."xsd:string |
| http://purl.uniprot.org/citations/19017395 | http://purl.uniprot.org/core/volume | "27"xsd:string |
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