| http://purl.uniprot.org/citations/19019087 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19019087 | http://www.w3.org/2000/01/rdf-schema#comment | "Interleukin-12 (IL-12) p70 (p40:p35) is a bioactive cytokine and its biological functions are becoming clear. On the other hand, the IL-12 p40 homodimer (p40(2)) was considered an inactive or inhibitory molecule and its functions are poorly understood. It has been reported that increased expression of lymphotoxin-alpha (Lt-alpha) in the central nervous system as well as in peripheral immune cells is associated with multiple sclerosis and experimental allergic encephalomyelitis. Here we describe that p40(2) induces the expression of Lt-alpha in primary mouse and human microglia, BV-2 microglial cells, splenic macrophages, RAW 264.7 cells and splenic T cells. Interestingly, IL-12 p70 was either unable to induce Lt-alpha or was a very weak inducer of Lt-alpha in these cell types. Consistently, p40(2), but not p70, induced Lt-alpha promoter-driven luciferase activity in microglial cells. Among various stimuli tested, p40(2) emerged as the most potent followed by IL-16, lipopolyaccharide and double-stranded RNA in inducing the activation of Lt-alpha promoter in microglial cells. Furthermore, an increase in Lt-alpha messenger RNA expression by overexpression of p40, but not p35, complementary DNA and induction of Lt-alpha expression by p40(2) in microglia isolated from IL-12p35(-/-) mice confirm that p40, but not p35, is responsible for the induction of Lt-alpha. Finally, by using primary microglia from IUL-12 receptor beta1 deficient (IL-12Rbeta1(-/-)) and IL-12Rbeta2(-/-) mice, we demonstrate that p40(2) induced the expression of Lt-alpha in microglia and macrophages via IL-12Rbeta1, but not IL-12Rbeta2. These studies delineate a novel biological function of p40(2) that is absent in IL-12."xsd:string |
| http://purl.uniprot.org/citations/19019087 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1365-2567.2008.02985.x"xsd:string |
| http://purl.uniprot.org/citations/19019087 | http://purl.uniprot.org/core/author | "Jana M."xsd:string |
| http://purl.uniprot.org/citations/19019087 | http://purl.uniprot.org/core/author | "Pahan K."xsd:string |
| http://purl.uniprot.org/citations/19019087 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19019087 | http://purl.uniprot.org/core/name | "Immunology"xsd:string |
| http://purl.uniprot.org/citations/19019087 | http://purl.uniprot.org/core/pages | "312-325"xsd:string |
| http://purl.uniprot.org/citations/19019087 | http://purl.uniprot.org/core/title | "Induction of lymphotoxin-alpha by interleukin-12 p40 homodimer, the so-called biologically inactive molecule, but not IL-12 p70."xsd:string |
| http://purl.uniprot.org/citations/19019087 | http://purl.uniprot.org/core/volume | "127"xsd:string |
| http://purl.uniprot.org/citations/19019087 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19019087 |
| http://purl.uniprot.org/citations/19019087 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19019087 |
| http://purl.uniprot.org/uniprot/#_F8WI71-mappedCitation-19019087 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19019087 |
| http://purl.uniprot.org/uniprot/#_Q3ZAX5-mappedCitation-19019087 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19019087 |
| http://purl.uniprot.org/uniprot/#_P43431-mappedCitation-19019087 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19019087 |
| http://purl.uniprot.org/uniprot/#_P43432-mappedCitation-19019087 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19019087 |
| http://purl.uniprot.org/uniprot/#_Q549G3-mappedCitation-19019087 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19019087 |
| http://purl.uniprot.org/uniprot/#_Q9QUT1-mappedCitation-19019087 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19019087 |
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| http://purl.uniprot.org/uniprot/Q549G3 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/19019087 |
| http://purl.uniprot.org/uniprot/P43432 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/19019087 |
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