| http://purl.uniprot.org/citations/19056676 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19056676 | http://www.w3.org/2000/01/rdf-schema#comment | "In previous reports, we have shown in SH-SY5 cells that olomoucine and roscovitine, two inhibitory drugs of cyclin-dependent kinases, caused apoptosis independent of the extrinsic pathway. In this experimental paradigm, apoptosis was refractory to the protective effects of either Bcl-2 or Bcl-XL overexpression. We are now reporting that the failure of Bcl-XL to prevent dell death was consistent with no effect on the kinetics of caspase activation and cytochrome c release. To further characterize this issue, we have discarded a direct effect of either olomoucine or roscovitine on mitochondrial permeability transition. Moreover, we have evidence that an intrinsic pathway took place in SH-SY5Y cells by showing the mitochondrial translocation of a GFP-Bax construct on transfection and treatment with cyclin-dependent kinase inhibitory drugs. Finally, we tested the effect of olomoucine and roscovitine on wild-type, bax-/-, bak-/-, and double bax-/-bak-/-mouse embryonic fibroblasts (MEF). In wild-type MEFs, both drugs induced cell death by apoptosis in a dose-dependent manner. In bax-/-, bak-/-, and, particularly, double bax-/-bak-/-MEFs, we observed the inhibition of apoptosis. In conclusion, olomoucine and roscovitine caused apoptosis through an intrinsic pathway, with Bax and Bak proteins being involved."xsd:string |
| http://purl.uniprot.org/citations/19056676 | http://purl.org/dc/terms/identifier | "doi:10.1158/1535-7163.mct-08-0655"xsd:string |
| http://purl.uniprot.org/citations/19056676 | http://purl.uniprot.org/core/author | "Jordan J."xsd:string |
| http://purl.uniprot.org/citations/19056676 | http://purl.uniprot.org/core/author | "Fernandez-Gomez F.J."xsd:string |
| http://purl.uniprot.org/citations/19056676 | http://purl.uniprot.org/core/author | "Melero-Fernandez de Mera R.M."xsd:string |
| http://purl.uniprot.org/citations/19056676 | http://purl.uniprot.org/core/author | "Boix J."xsd:string |
| http://purl.uniprot.org/citations/19056676 | http://purl.uniprot.org/core/author | "Garrofe-Ochoa X."xsd:string |
| http://purl.uniprot.org/citations/19056676 | http://purl.uniprot.org/core/author | "Ribas J."xsd:string |
| http://purl.uniprot.org/citations/19056676 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/19056676 | http://purl.uniprot.org/core/name | "Mol Cancer Ther"xsd:string |
| http://purl.uniprot.org/citations/19056676 | http://purl.uniprot.org/core/pages | "3800-3806"xsd:string |
| http://purl.uniprot.org/citations/19056676 | http://purl.uniprot.org/core/title | "BAX and BAK proteins are required for cyclin-dependent kinase inhibitory drugs to cause apoptosis."xsd:string |
| http://purl.uniprot.org/citations/19056676 | http://purl.uniprot.org/core/volume | "7"xsd:string |
| http://purl.uniprot.org/citations/19056676 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19056676 |
| http://purl.uniprot.org/citations/19056676 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19056676 |
| http://purl.uniprot.org/uniprot/#_A0A0S2Z319-mappedCitation-19056676 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19056676 |
| http://purl.uniprot.org/uniprot/#_A0A165ELP6-mappedCitation-19056676 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19056676 |
| http://purl.uniprot.org/uniprot/#_Q07813-mappedCitation-19056676 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19056676 |
| http://purl.uniprot.org/uniprot/#_A0A0S2Z391-mappedCitation-19056676 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19056676 |
| http://purl.uniprot.org/uniprot/#_B3KRK7-mappedCitation-19056676 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19056676 |
| http://purl.uniprot.org/uniprot/#_A8K7S8-mappedCitation-19056676 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19056676 |
| http://purl.uniprot.org/uniprot/#_B4DEB4-mappedCitation-19056676 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19056676 |
| http://purl.uniprot.org/uniprot/#_B4E0L2-mappedCitation-19056676 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19056676 |
| http://purl.uniprot.org/uniprot/#_Q16611-mappedCitation-19056676 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19056676 |