| http://purl.uniprot.org/citations/19064640 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19064640 | http://www.w3.org/2000/01/rdf-schema#comment | "Wrch-1, an atypical and transforming Rho GTPase, regulates cellular activities including proliferation and actin organization, but its functions and effectors remain poorly characterized. We show here that Wrch-1 distributes along the apical and basolateral membranes in MDCK cells and binds the cell polarity protein Par6 in a GTP-dependent manner. Activated Wrch-1 negatively regulates the kinetics of tight junction (TJ) assembly during epithelial cell polarization but has no detectable effect on overall cell polarity in confluent monolayers. It also causes a dramatic cytoskeletal reorganization and multilayering in cells grown in two-dimensional culture and disrupts cystogenesis of cells grown in three-dimensional (3D) culture. Similarly, short hairpin RNA-mediated knockdown of Wrch-1 perturbs cystogenesis in 3D culture, suggesting that tight regulation of Wrch-1 activity is necessary for normal epithelial morphogenesis. A weakly transforming effector domain mutant of activated Wrch-1 that inhibits Par6 binding abrogates the ability of Wrch-1 to disrupt TJ formation, actin organization, and epithelial morphogenesis. We hypothesize that Wrch-1-induced morphological and growth transformation may occur in part through Par6-mediated disruption of TJs and actin organization."xsd:string |
| http://purl.uniprot.org/citations/19064640 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.00336-08"xsd:string |
| http://purl.uniprot.org/citations/19064640 | http://purl.uniprot.org/core/author | "Fanning A.S."xsd:string |
| http://purl.uniprot.org/citations/19064640 | http://purl.uniprot.org/core/author | "Madigan J.P."xsd:string |
| http://purl.uniprot.org/citations/19064640 | http://purl.uniprot.org/core/author | "Cox A.D."xsd:string |
| http://purl.uniprot.org/citations/19064640 | http://purl.uniprot.org/core/author | "Brady D.C."xsd:string |
| http://purl.uniprot.org/citations/19064640 | http://purl.uniprot.org/core/author | "Alan J.K."xsd:string |
| http://purl.uniprot.org/citations/19064640 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19064640 | http://purl.uniprot.org/core/name | "Mol Cell Biol"xsd:string |
| http://purl.uniprot.org/citations/19064640 | http://purl.uniprot.org/core/pages | "1035-1049"xsd:string |
| http://purl.uniprot.org/citations/19064640 | http://purl.uniprot.org/core/title | "The transforming Rho family GTPase Wrch-1 disrupts epithelial cell tight junctions and epithelial morphogenesis."xsd:string |
| http://purl.uniprot.org/citations/19064640 | http://purl.uniprot.org/core/volume | "29"xsd:string |
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