| http://purl.uniprot.org/citations/19074020 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19074020 | http://www.w3.org/2000/01/rdf-schema#comment | "In the injured spinal cord, a glial scar forms and becomes a major obstacle to axonal regeneration. Formation of the glial scar involves migration of astrocytes toward the lesion. Matrix metalloproteinases (MMPs), including MMP-9 and MMP-2, govern cell migration through their ability to degrade constituents of the extracellular matrix. Although MMP-9 is expressed in reactive astrocytes, its involvement in astrocyte migration and formation of a glial scar is unknown. Here we found that spinal cord injured, wild-type mice expressing MMPs developed a more severe glial scar and enhanced expression of chondroitin sulfate proteoglycans, indicative of a more inhibitory environment for axonal regeneration/plasticity, than MMP-9 null mice. To determine whether MMP-9 mediates astrocyte migration, we conducted a scratch wound assay using astrocytes cultured from MMP-9 null, MMP-2 null, and wild-type mice. Gelatin zymography confirmed the expression of MMP-9 and MMP-2 in wild-type cultures. MMP-9 null astrocytes and wild-type astrocytes, treated with an MMP-9 inhibitor, exhibited impaired migration relative to untreated wild-type controls. MMP-9 null astrocytes showed abnormalities in the actin cytoskeletal organization and function but no detectable untoward effects on proliferation, cellular viability, or adhesion. Interestingly, MMP-2 null astrocytes showed increased migration, which could be attenuated in the presence of an MMP-9 inhibitor. Collectively, our studies provide explicit evidence that MMP-9 is integral to the formation of an inhibitory glial scar and cytoskeleton-mediated astrocyte migration. MMP-9 may thus be a promising therapeutic target to reduce glial scarring during wound healing after spinal cord injury."xsd:string |
| http://purl.uniprot.org/citations/19074020 | http://purl.org/dc/terms/identifier | "doi:10.1523/jneurosci.2287-08.2008"xsd:string |
| http://purl.uniprot.org/citations/19074020 | http://purl.uniprot.org/core/author | "Zhang H."xsd:string |
| http://purl.uniprot.org/citations/19074020 | http://purl.uniprot.org/core/author | "Peyrollier K."xsd:string |
| http://purl.uniprot.org/citations/19074020 | http://purl.uniprot.org/core/author | "Bourguignon L.Y."xsd:string |
| http://purl.uniprot.org/citations/19074020 | http://purl.uniprot.org/core/author | "Werb Z."xsd:string |
| http://purl.uniprot.org/citations/19074020 | http://purl.uniprot.org/core/author | "Hsu J.Y."xsd:string |
| http://purl.uniprot.org/citations/19074020 | http://purl.uniprot.org/core/author | "Adams C.M."xsd:string |
| http://purl.uniprot.org/citations/19074020 | http://purl.uniprot.org/core/author | "Noble-Haeusslein L.J."xsd:string |
| http://purl.uniprot.org/citations/19074020 | http://purl.uniprot.org/core/author | "Fandel T."xsd:string |
| http://purl.uniprot.org/citations/19074020 | http://purl.uniprot.org/core/author | "Cun C.L."xsd:string |
| http://purl.uniprot.org/citations/19074020 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/19074020 | http://purl.uniprot.org/core/name | "J Neurosci"xsd:string |
| http://purl.uniprot.org/citations/19074020 | http://purl.uniprot.org/core/pages | "13467-13477"xsd:string |
| http://purl.uniprot.org/citations/19074020 | http://purl.uniprot.org/core/title | "Matrix metalloproteinase-9 facilitates glial scar formation in the injured spinal cord."xsd:string |
| http://purl.uniprot.org/citations/19074020 | http://purl.uniprot.org/core/volume | "28"xsd:string |
| http://purl.uniprot.org/citations/19074020 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19074020 |
| http://purl.uniprot.org/citations/19074020 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19074020 |
| http://purl.uniprot.org/uniprot/#_A2A5K8-mappedCitation-19074020 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19074020 |
| http://purl.uniprot.org/uniprot/#_A0A1B0GS88-mappedCitation-19074020 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19074020 |
| http://purl.uniprot.org/uniprot/#_P41245-mappedCitation-19074020 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19074020 |
| http://purl.uniprot.org/uniprot/#_Q2M4J5-mappedCitation-19074020 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19074020 |
| http://purl.uniprot.org/uniprot/#_Q3TTU7-mappedCitation-19074020 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19074020 |
| http://purl.uniprot.org/uniprot/#_Q3UG07-mappedCitation-19074020 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19074020 |