| http://purl.uniprot.org/citations/19109425 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19109425 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19109425 | http://www.w3.org/2000/01/rdf-schema#comment | "Yeast and animal homotypic fusion and vacuole protein sorting (HOPS) complexes contain conserved subunits, but HOPS-mediated traffic in animals might require additional proteins. Here, we demonstrate that SPE-39 homologues, which are found only in animals, are present in RAB5-, RAB7-, and RAB11-positive endosomes where they play a conserved role in lysosomal delivery and probably function via their interaction with the core HOPS complex. Although Caenorhabditis elegans spe-39 mutants were initially identified as having abnormal vesicular biogenesis during spermatogenesis, we show that these mutants also have disrupted processing of endocytosed proteins in oocytes and coelomocytes. C. elegans SPE-39 interacts in vitro with both VPS33A and VPS33B, whereas RNA interference of VPS33B causes spe-39-like spermatogenesis defects. The human SPE-39 orthologue C14orf133 also interacts with VPS33 homologues and both coimmunoprecipitates and cosediments with other HOPS subunits. SPE-39 knockdown in cultured human cells altered the morphology of syntaxin 7-, syntaxin 8-, and syntaxin 13-positive endosomes. These effects occurred concomitantly with delayed mannose 6-phosphate receptor-mediated cathepsin D delivery and degradation of internalized epidermal growth factor receptors. Our findings establish that SPE-39 proteins are a previously unrecognized regulator of lysosomal delivery and that C. elegans spermatogenesis is an experimental system useful for identifying conserved regulators of metazoan lysosomal biogenesis."xsd:string |
| http://purl.uniprot.org/citations/19109425 | http://purl.org/dc/terms/identifier | "doi:10.1091/mbc.e08-07-0728"xsd:string |
| http://purl.uniprot.org/citations/19109425 | http://purl.org/dc/terms/identifier | "doi:10.1091/mbc.e08-07-0728"xsd:string |
| http://purl.uniprot.org/citations/19109425 | http://purl.uniprot.org/core/author | "Levey A.I."xsd:string |
| http://purl.uniprot.org/citations/19109425 | http://purl.uniprot.org/core/author | "Levey A.I."xsd:string |
| http://purl.uniprot.org/citations/19109425 | http://purl.uniprot.org/core/author | "Faundez V."xsd:string |
| http://purl.uniprot.org/citations/19109425 | http://purl.uniprot.org/core/author | "Faundez V."xsd:string |
| http://purl.uniprot.org/citations/19109425 | http://purl.uniprot.org/core/author | "Salazar G."xsd:string |
| http://purl.uniprot.org/citations/19109425 | http://purl.uniprot.org/core/author | "Salazar G."xsd:string |
| http://purl.uniprot.org/citations/19109425 | http://purl.uniprot.org/core/author | "Zlatic S.A."xsd:string |
| http://purl.uniprot.org/citations/19109425 | http://purl.uniprot.org/core/author | "Zlatic S.A."xsd:string |
| http://purl.uniprot.org/citations/19109425 | http://purl.uniprot.org/core/author | "Doucette M.M."xsd:string |
| http://purl.uniprot.org/citations/19109425 | http://purl.uniprot.org/core/author | "Doucette M.M."xsd:string |
| http://purl.uniprot.org/citations/19109425 | http://purl.uniprot.org/core/author | "L'hernault S.W."xsd:string |
| http://purl.uniprot.org/citations/19109425 | http://purl.uniprot.org/core/author | "L'hernault S.W."xsd:string |
| http://purl.uniprot.org/citations/19109425 | http://purl.uniprot.org/core/author | "Heilman C.J."xsd:string |
| http://purl.uniprot.org/citations/19109425 | http://purl.uniprot.org/core/author | "Heilman C.J."xsd:string |
| http://purl.uniprot.org/citations/19109425 | http://purl.uniprot.org/core/author | "Fiza B."xsd:string |
| http://purl.uniprot.org/citations/19109425 | http://purl.uniprot.org/core/author | "Fiza B."xsd:string |
| http://purl.uniprot.org/citations/19109425 | http://purl.uniprot.org/core/author | "Zhu G.D."xsd:string |
| http://purl.uniprot.org/citations/19109425 | http://purl.uniprot.org/core/author | "Zhu G.D."xsd:string |
| http://purl.uniprot.org/citations/19109425 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19109425 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |