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http://purl.uniprot.org/citations/19118587http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/19118587http://www.w3.org/2000/01/rdf-schema#comment"PBCV-1 (Paramecium bursaria Chlorella virus) is a large double stranded DNA virus that replicates in certain eukaryotic chlorella like green algae. The PBCV-1 A312L gene encodes a 33-kDa protein whose function currently is unknown. The 5'-UTR of the A312L mRNA is 153 nucleotides, longer than the 5'-UTR in any other PBCV-1 gene. The sequence 5'-AAAC was repeated 17 times within 156bp 5' to the A312L gene start codon and this sequence was repeated 13 times continuously in the 5'-UTR of the mRNA. Recombinant genes were constructed in vector pBI121 that contained the A312L 5'-UTR, in both the forward and inverse-complement orientations, fused to the GUS gene under the control of the CaMV 35S promoter. These constructs were introduced into Arabidopsis thaliana and the results indicated that the A312L 5'-UTR functions as a translational enhancer only in the forward orientation. Overall, the ratio of GUS enzyme activity to GUS mRNA was 15-fold higher in constructs derived from the A312L 5'-UTR in the forward orientation as compared to constructs containing the 5'-UTR in the inverse-complement orientation or those lacking the A312L 5'-UTR."xsd:string
http://purl.uniprot.org/citations/19118587http://purl.org/dc/terms/identifier"doi:10.1016/j.virusres.2008.11.017"xsd:string
http://purl.uniprot.org/citations/19118587http://purl.uniprot.org/core/author"Falcone D.L."xsd:string
http://purl.uniprot.org/citations/19118587http://purl.uniprot.org/core/author"Graves M.V."xsd:string
http://purl.uniprot.org/citations/19118587http://purl.uniprot.org/core/author"Nguyen P.S."xsd:string
http://purl.uniprot.org/citations/19118587http://purl.uniprot.org/core/date"2009"xsd:gYear
http://purl.uniprot.org/citations/19118587http://purl.uniprot.org/core/name"Virus Res"xsd:string
http://purl.uniprot.org/citations/19118587http://purl.uniprot.org/core/pages"138-146"xsd:string
http://purl.uniprot.org/citations/19118587http://purl.uniprot.org/core/title"The A312L 5'-UTR of Chlorella virus PBCV-1 is a translational enhancer in Arabidopsis thaliana."xsd:string
http://purl.uniprot.org/citations/19118587http://purl.uniprot.org/core/volume"140"xsd:string
http://purl.uniprot.org/citations/19118587http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/19118587
http://purl.uniprot.org/citations/19118587http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/19118587
http://purl.uniprot.org/uniprot/#_Q9FMD8-mappedCitation-19118587http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19118587
http://purl.uniprot.org/uniprot/Q9FMD8http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/19118587