http://purl.uniprot.org/citations/19118587 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/19118587 | http://www.w3.org/2000/01/rdf-schema#comment | "PBCV-1 (Paramecium bursaria Chlorella virus) is a large double stranded DNA virus that replicates in certain eukaryotic chlorella like green algae. The PBCV-1 A312L gene encodes a 33-kDa protein whose function currently is unknown. The 5'-UTR of the A312L mRNA is 153 nucleotides, longer than the 5'-UTR in any other PBCV-1 gene. The sequence 5'-AAAC was repeated 17 times within 156bp 5' to the A312L gene start codon and this sequence was repeated 13 times continuously in the 5'-UTR of the mRNA. Recombinant genes were constructed in vector pBI121 that contained the A312L 5'-UTR, in both the forward and inverse-complement orientations, fused to the GUS gene under the control of the CaMV 35S promoter. These constructs were introduced into Arabidopsis thaliana and the results indicated that the A312L 5'-UTR functions as a translational enhancer only in the forward orientation. Overall, the ratio of GUS enzyme activity to GUS mRNA was 15-fold higher in constructs derived from the A312L 5'-UTR in the forward orientation as compared to constructs containing the 5'-UTR in the inverse-complement orientation or those lacking the A312L 5'-UTR."xsd:string |
http://purl.uniprot.org/citations/19118587 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.virusres.2008.11.017"xsd:string |
http://purl.uniprot.org/citations/19118587 | http://purl.uniprot.org/core/author | "Falcone D.L."xsd:string |
http://purl.uniprot.org/citations/19118587 | http://purl.uniprot.org/core/author | "Graves M.V."xsd:string |
http://purl.uniprot.org/citations/19118587 | http://purl.uniprot.org/core/author | "Nguyen P.S."xsd:string |
http://purl.uniprot.org/citations/19118587 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
http://purl.uniprot.org/citations/19118587 | http://purl.uniprot.org/core/name | "Virus Res"xsd:string |
http://purl.uniprot.org/citations/19118587 | http://purl.uniprot.org/core/pages | "138-146"xsd:string |
http://purl.uniprot.org/citations/19118587 | http://purl.uniprot.org/core/title | "The A312L 5'-UTR of Chlorella virus PBCV-1 is a translational enhancer in Arabidopsis thaliana."xsd:string |
http://purl.uniprot.org/citations/19118587 | http://purl.uniprot.org/core/volume | "140"xsd:string |
http://purl.uniprot.org/citations/19118587 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19118587 |
http://purl.uniprot.org/citations/19118587 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19118587 |
http://purl.uniprot.org/uniprot/#_Q9FMD8-mappedCitation-19118587 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19118587 |
http://purl.uniprot.org/uniprot/Q9FMD8 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/19118587 |