| http://purl.uniprot.org/citations/19119907 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19119907 | http://www.w3.org/2000/01/rdf-schema#comment | "ObjectMedulloblastoma (MB) is a malignant embryonal tumor of the cerebellum. Amplification of c-myc or N-myc is infrequently identified and, when present, is often associated with the large cell/anaplastic (LC/A) phenotype. The frequency of low-level copy gain of myc oncogenes and its relationship to prognosis of MB has not been explored.MethodsArchival cases of MB were histologically reviewed and classified into 3 major subtypes: classic, nodular, and LC/A. Using quantitative real-time polymerase chain reaction (PCR), the authors analyzed 58 cases with a pure histological subtype for the copy number (CN) of myc (c-myc and N-myc) oncogenes. Cases with > 5-fold CN were further analyzed using the fluorescent in situ hybridization (FISH) assay. Kaplan-Meier survival analysis was performed.ResultsA > 5-fold myc CN was noted in 5 (20.8%) of 24 LC/A, 1 (5.3%) of 19 classic, and 2 (13.3%) of 15 nodular subtypes. In a significant number of tumors (14 [56%] of 24 LC/A, 13 [68%] of 19 classic, and 10 [67%] of 15 nodular MBs) the CN was > 2-fold but < 5-fold. High-level amplification, defined as > 10-fold CN, was only seen in the LC/A subtype (5 cases), although moderate amplification (> 5-fold but < 10-fold) could be detected in other histological subtypes. Fluorescence in situ hybridization readily detected most cases corresponding to tumors with > 5-fold amplicon CN by quantitative real-time PCR, and could detect all 5 cases with > 10-fold CN by quantitative real-time PCR. The group of patients with > 5-fold myc amplicon CN showed significantly shorter survival than those with < 5-fold CN (p = 0.045), independent of histological subtype.ConclusionsSince FISH could easily detect most cases in the moderate-to-high myc gene amplification (> 5-fold CN) group, the FISH assay has utility in detecting subsets of MB with poorer prognosis."xsd:string |
| http://purl.uniprot.org/citations/19119907 | http://purl.org/dc/terms/identifier | "doi:10.3171/2008.10.peds08105"xsd:string |
| http://purl.uniprot.org/citations/19119907 | http://purl.uniprot.org/core/author | "Takei H."xsd:string |
| http://purl.uniprot.org/citations/19119907 | http://purl.uniprot.org/core/author | "Nguyen Y."xsd:string |
| http://purl.uniprot.org/citations/19119907 | http://purl.uniprot.org/core/author | "Adesina A.M."xsd:string |
| http://purl.uniprot.org/citations/19119907 | http://purl.uniprot.org/core/author | "Chintagumpala M."xsd:string |
| http://purl.uniprot.org/citations/19119907 | http://purl.uniprot.org/core/author | "Mehta V."xsd:string |
| http://purl.uniprot.org/citations/19119907 | http://purl.uniprot.org/core/author | "Dauser R.C."xsd:string |
| http://purl.uniprot.org/citations/19119907 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19119907 | http://purl.uniprot.org/core/name | "J Neurosurg Pediatr"xsd:string |
| http://purl.uniprot.org/citations/19119907 | http://purl.uniprot.org/core/pages | "61-65"xsd:string |
| http://purl.uniprot.org/citations/19119907 | http://purl.uniprot.org/core/title | "Low-level copy gain versus amplification of myc oncogenes in medulloblastoma: utility in predicting prognosis and survival. Laboratory investigation."xsd:string |
| http://purl.uniprot.org/citations/19119907 | http://purl.uniprot.org/core/volume | "3"xsd:string |
| http://purl.uniprot.org/citations/19119907 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19119907 |
| http://purl.uniprot.org/citations/19119907 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19119907 |
| http://purl.uniprot.org/uniprot/#_A0A0B4J1R1-mappedCitation-19119907 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19119907 |
| http://purl.uniprot.org/uniprot/#_A0N2G3-mappedCitation-19119907 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19119907 |
| http://purl.uniprot.org/uniprot/#_A0A224B085-mappedCitation-19119907 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19119907 |
| http://purl.uniprot.org/uniprot/#_B3CJ86-mappedCitation-19119907 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19119907 |
| http://purl.uniprot.org/uniprot/#_B3CJ87-mappedCitation-19119907 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19119907 |
| http://purl.uniprot.org/uniprot/#_B3CJ88-mappedCitation-19119907 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19119907 |
| http://purl.uniprot.org/uniprot/#_B3CJB3-mappedCitation-19119907 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19119907 |
| http://purl.uniprot.org/uniprot/#_B3CJB8-mappedCitation-19119907 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19119907 |
| http://purl.uniprot.org/uniprot/#_B3CJ63-mappedCitation-19119907 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19119907 |