| http://purl.uniprot.org/citations/19126643 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19126643 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19126643 | http://www.w3.org/2000/01/rdf-schema#comment | "Human prostate cancer (PCa) and prostate epithelial cells predominantly express estrogen receptor (ER) beta, but not ERalpha. ERbeta might utilize various ER coregulators to mediate the E2-signaling pathway in PCa. Here, we identified coiled-coil domain containing 62 (CCDC62)/ERAP75 as a novel ER coactivator. CCDC62/ERAP75 is widely expressed in PCa cell lines and has low expression in MCF7 cells. Both in vitro and in vivo interaction assays using mammalian two-hybrid, glutathione S-transferase pull-down and coimmunoprecipitation methods proved that ERbeta can interact with the C-terminus of CCDC62/ERAP75 via the ligand-binding domain. The first LXXLL motif within CCDC62/ERAP75 is required for the interaction between ERbeta and CCDC62/ERAP75. Electrophoretic mobility shift assay showed that CCDC62/ERAP75 can be recruited by the estrogen response element-ER complex in the presence of ligand. Furthermore, a chromatin immunoprecipitation assay demonstrated the hormone-dependent recruitment of CCDC62/ERAP75 within the promoter of the estrogen-responsive gene cyclin D1. In addition, using silencing RNA (siRNA) against endogeneous CCDC62/ERAP75, we demonstrated that inhibition of endogenous CCDC62/ERAP75 results in the suppression of ERbeta-mediated transactivation as well as target gene expression in LNCaP cells. More importantly, using the tet-on overexpression system, we showed that induced expression of CCDC62/ERAP75 can enhance the E2-regulated cyclin D1 expression and cell growth in LNCaP cells. Together, our results revealed the role of CCDC62/ERAP75 as a novel coactivator in PCa cells that can modulate ERbeta transactivation and receptor function."xsd:string |
| http://purl.uniprot.org/citations/19126643 | http://purl.org/dc/terms/identifier | "doi:10.1093/carcin/bgn288"xsd:string |
| http://purl.uniprot.org/citations/19126643 | http://purl.org/dc/terms/identifier | "doi:10.1093/carcin/bgn288"xsd:string |
| http://purl.uniprot.org/citations/19126643 | http://purl.uniprot.org/core/author | "Chen M."xsd:string |
| http://purl.uniprot.org/citations/19126643 | http://purl.uniprot.org/core/author | "Chen M."xsd:string |
| http://purl.uniprot.org/citations/19126643 | http://purl.uniprot.org/core/author | "Chang H.C."xsd:string |
| http://purl.uniprot.org/citations/19126643 | http://purl.uniprot.org/core/author | "Chang H.C."xsd:string |
| http://purl.uniprot.org/citations/19126643 | http://purl.uniprot.org/core/author | "Lin C.Y."xsd:string |
| http://purl.uniprot.org/citations/19126643 | http://purl.uniprot.org/core/author | "Lin C.Y."xsd:string |
| http://purl.uniprot.org/citations/19126643 | http://purl.uniprot.org/core/author | "Ni J."xsd:string |
| http://purl.uniprot.org/citations/19126643 | http://purl.uniprot.org/core/author | "Ni J."xsd:string |
| http://purl.uniprot.org/citations/19126643 | http://purl.uniprot.org/core/author | "Yeh S."xsd:string |
| http://purl.uniprot.org/citations/19126643 | http://purl.uniprot.org/core/author | "Yeh S."xsd:string |
| http://purl.uniprot.org/citations/19126643 | http://purl.uniprot.org/core/author | "Muyan M."xsd:string |
| http://purl.uniprot.org/citations/19126643 | http://purl.uniprot.org/core/author | "Muyan M."xsd:string |
| http://purl.uniprot.org/citations/19126643 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19126643 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19126643 | http://purl.uniprot.org/core/name | "Carcinogenesis"xsd:string |
| http://purl.uniprot.org/citations/19126643 | http://purl.uniprot.org/core/name | "Carcinogenesis"xsd:string |
| http://purl.uniprot.org/citations/19126643 | http://purl.uniprot.org/core/pages | "841-850"xsd:string |
| http://purl.uniprot.org/citations/19126643 | http://purl.uniprot.org/core/pages | "841-850"xsd:string |
| http://purl.uniprot.org/citations/19126643 | http://purl.uniprot.org/core/title | "CCDC62/ERAP75 functions as a coactivator to enhance estrogen receptor beta-mediated transactivation and target gene expression in prostate cancer cells."xsd:string |
| http://purl.uniprot.org/citations/19126643 | http://purl.uniprot.org/core/title | "CCDC62/ERAP75 functions as a coactivator to enhance estrogen receptor beta-mediated transactivation and target gene expression in prostate cancer cells."xsd:string |