| http://purl.uniprot.org/citations/19129223 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19129223 | http://www.w3.org/2000/01/rdf-schema#comment | "Regulation of expression of the CFTR gene is poorly understood. Elements within the basal promoter of the gene do not fully explain CFTR expression patterns, suggesting that cis-regulatory elements are located elsewhere, either within the locus or in adjacent chromatin. We previously mapped DNase I hypersensitive sites (DHS) in 400 kb spanning the CFTR locus including a cluster of sites close to the 3'-end of the gene. Here we focus on a DHS at +6.8 kb from the CFTR translation end-point to evaluate its potential role in regulating expression of the gene. This DHS, which encompasses a consensus CTCF-binding site, was evident in primary human epididymis cells that express abundant CFTR mRNA. We show by DNase I footprinting and electophoretic mobility shift assays that the cis-regulatory element within this DHS binds CTCF in vitro. We further demonstrate that the element functions as an enhancer blocker in a well-established in vivo assay, and by using chromatin immunoprecipitation that it recruits CTCF in vivo. Moreover, we reveal that in primary epididymis cells, the +6.8 kb DHS interacts closely with the CFTR promoter, suggesting that the CFTR locus exists in a looped conformation, characteristic of an active chromatin hub."xsd:string |
| http://purl.uniprot.org/citations/19129223 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/gkn1056"xsd:string |
| http://purl.uniprot.org/citations/19129223 | http://purl.uniprot.org/core/author | "Harris A."xsd:string |
| http://purl.uniprot.org/citations/19129223 | http://purl.uniprot.org/core/author | "Blackledge N.P."xsd:string |
| http://purl.uniprot.org/citations/19129223 | http://purl.uniprot.org/core/author | "Gillen A.E."xsd:string |
| http://purl.uniprot.org/citations/19129223 | http://purl.uniprot.org/core/author | "Ott C.J."xsd:string |
| http://purl.uniprot.org/citations/19129223 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19129223 | http://purl.uniprot.org/core/name | "Nucleic Acids Res"xsd:string |
| http://purl.uniprot.org/citations/19129223 | http://purl.uniprot.org/core/pages | "1086-1094"xsd:string |
| http://purl.uniprot.org/citations/19129223 | http://purl.uniprot.org/core/title | "An insulator element 3' to the CFTR gene binds CTCF and reveals an active chromatin hub in primary cells."xsd:string |
| http://purl.uniprot.org/citations/19129223 | http://purl.uniprot.org/core/volume | "37"xsd:string |
| http://purl.uniprot.org/citations/19129223 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19129223 |
| http://purl.uniprot.org/citations/19129223 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19129223 |
| http://purl.uniprot.org/uniprot/#_A0A089NFM8-mappedCitation-19129223 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19129223 |
| http://purl.uniprot.org/uniprot/#_A0A089PPW6-mappedCitation-19129223 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19129223 |
| http://purl.uniprot.org/uniprot/#_A0A088T1A1-mappedCitation-19129223 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19129223 |
| http://purl.uniprot.org/uniprot/#_A0A096ZUB9-mappedCitation-19129223 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19129223 |
| http://purl.uniprot.org/uniprot/#_A0A096ZUC0-mappedCitation-19129223 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19129223 |
| http://purl.uniprot.org/uniprot/#_A0A096ZUC4-mappedCitation-19129223 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19129223 |
| http://purl.uniprot.org/uniprot/#_A0A096ZUH4-mappedCitation-19129223 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19129223 |
| http://purl.uniprot.org/uniprot/#_A0A096ZUB3-mappedCitation-19129223 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19129223 |
| http://purl.uniprot.org/uniprot/#_A0A096ZUB7-mappedCitation-19129223 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19129223 |
| http://purl.uniprot.org/uniprot/#_A0A096ZUB8-mappedCitation-19129223 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19129223 |
| http://purl.uniprot.org/uniprot/#_A0A1B0YU32-mappedCitation-19129223 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19129223 |