| http://purl.uniprot.org/citations/19132534 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19132534 | http://www.w3.org/2000/01/rdf-schema#comment | "Although the binding of synaphin (also called complexin) to the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex is critical for synaptic vesicle exocytosis, the exact role of synaphin remains unclear. Here, we show that synaphin directly binds to synaptotagmin 1, a major Ca(2+) sensor for fast neurotransmitter release, in a 1:1 stoichiometry. Mapping of the synaphin site involved in synaptotagmin 1 binding revealed that the C-terminal region is essential for the interaction between these two proteins. Binding was sensitive to ionic strength, suggesting the involvement of charged residues in the C-terminus region. Mutation of the seven consecutive glutamic acid residues (residues 108-114) at the C-terminal region of synaphin to alanines or glutamines resulted in a dramatic reduction in synaptotagmin 1 binding activity. Furthermore, a peptide from the C-terminus of synaphin (residues 91-124) blocked the binding of synaptotagmin 1 to synaphin, an effect that was abolished by mutating the consecutive glutamic acid residues to alanine. Immunoprecipitation experiments with brain membrane extracts showed the presence of a complex consisting of synaphin, synaptotagmin 1, and SNAREs. We propose that synaphin recruits synaptotagmin 1 to the SNARE-based fusion complex and synergistically functions with synaptotagmin 1 in mediating fast synaptic vesicle exocytosis."xsd:string |
| http://purl.uniprot.org/citations/19132534 | http://purl.org/dc/terms/identifier | "doi:10.1007/s11068-008-9032-9"xsd:string |
| http://purl.uniprot.org/citations/19132534 | http://purl.uniprot.org/core/author | "Abe T."xsd:string |
| http://purl.uniprot.org/citations/19132534 | http://purl.uniprot.org/core/author | "Tokumaru H."xsd:string |
| http://purl.uniprot.org/citations/19132534 | http://purl.uniprot.org/core/author | "Shimizu-Okabe C."xsd:string |
| http://purl.uniprot.org/citations/19132534 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/19132534 | http://purl.uniprot.org/core/name | "Brain Cell Biol"xsd:string |
| http://purl.uniprot.org/citations/19132534 | http://purl.uniprot.org/core/pages | "173-189"xsd:string |
| http://purl.uniprot.org/citations/19132534 | http://purl.uniprot.org/core/title | "Direct interaction of SNARE complex binding protein synaphin/complexin with calcium sensor synaptotagmin 1."xsd:string |
| http://purl.uniprot.org/citations/19132534 | http://purl.uniprot.org/core/volume | "36"xsd:string |
| http://purl.uniprot.org/citations/19132534 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19132534 |
| http://purl.uniprot.org/citations/19132534 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19132534 |
| http://purl.uniprot.org/uniprot/P84087#attribution-11A7A7B4EE8E6B28DCCC9D6A1A8BC0E9 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/19132534 |
| http://purl.uniprot.org/uniprot/P84087#attribution-D5F7E1FB2507535885A223C2AF302563 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/19132534 |
| http://purl.uniprot.org/uniprot/P60881#attribution-D5F7E1FB2507535885A223C2AF302563 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/19132534 |
| http://purl.uniprot.org/uniprot/P32851#attribution-D5F7E1FB2507535885A223C2AF302563 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/19132534 |
| http://purl.uniprot.org/uniprot/P63045#attribution-D5F7E1FB2507535885A223C2AF302563 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/19132534 |
| http://purl.uniprot.org/uniprot/#_A0A0G2K3F2-mappedCitation-19132534 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19132534 |
| http://purl.uniprot.org/uniprot/#_D4A5W9-mappedCitation-19132534 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19132534 |
| http://purl.uniprot.org/uniprot/#_P63041-mappedCitation-19132534 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19132534 |
| http://purl.uniprot.org/uniprot/#_P63045-mappedCitation-19132534 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19132534 |
| http://purl.uniprot.org/uniprot/#_A6IGE2-mappedCitation-19132534 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19132534 |
| http://purl.uniprot.org/uniprot/#_A0A8I6AQV6-mappedCitation-19132534 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19132534 |
| http://purl.uniprot.org/uniprot/#_A0A8I6AVN2-mappedCitation-19132534 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19132534 |