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http://purl.uniprot.org/citations/19133617http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/19133617http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/19133617http://www.w3.org/2000/01/rdf-schema#comment"

Objective

To identify waterborne enteric viruses in Korean surface water.

Methods

Integrated cell culture (ICC)-multiplex reverse transcription-polymerase chain reaction (RT-PCR) was simultaneously designed to detect coxsackieviruses (CV), polioviruses (PV), and reoviruses (RV). ICC-multiplex RT-PCR and phylogenetic analysis were conducted using 21 total culturable virus assay (TCVA)-positive sample-inoculated cell cultures.

Results

CV and RV were detected in 9 samples each, and 3 samples were positive for both CV and RV. PV was not detected in any sample. Molecular phylogenetic analysis of the VP1 gene sequences revealed that CV types B2 and B4 predominated in Korean surface water, and the nucleotide sequences of CV type B2 were clustered with those of CVs isolated from China and Japan. The results suggested that the evolution of these viruses occurred in a region-specific manner.

Conclusion

CV and RV are detectable in Korean surface water, with a predominance of CV type B2, and the evolution of CV type B2 occur in a region-specific manner."xsd:string
http://purl.uniprot.org/citations/19133617http://purl.org/dc/terms/identifier"doi:10.1016/s0895-3988(08)60064-1"xsd:string
http://purl.uniprot.org/citations/19133617http://purl.uniprot.org/core/author"Lee G."xsd:string
http://purl.uniprot.org/citations/19133617http://purl.uniprot.org/core/author"Lee G."xsd:string
http://purl.uniprot.org/citations/19133617http://purl.uniprot.org/core/author"Lee C."xsd:string
http://purl.uniprot.org/citations/19133617http://purl.uniprot.org/core/author"Lee C."xsd:string
http://purl.uniprot.org/citations/19133617http://purl.uniprot.org/core/author"Park C."xsd:string
http://purl.uniprot.org/citations/19133617http://purl.uniprot.org/core/author"Park C."xsd:string
http://purl.uniprot.org/citations/19133617http://purl.uniprot.org/core/author"Jeong S."xsd:string
http://purl.uniprot.org/citations/19133617http://purl.uniprot.org/core/author"Jeong S."xsd:string
http://purl.uniprot.org/citations/19133617http://purl.uniprot.org/core/date"2008"xsd:gYear
http://purl.uniprot.org/citations/19133617http://purl.uniprot.org/core/date"2008"xsd:gYear
http://purl.uniprot.org/citations/19133617http://purl.uniprot.org/core/name"Biomed. Environ. Sci."xsd:string
http://purl.uniprot.org/citations/19133617http://purl.uniprot.org/core/name"Biomed Environ Sci"xsd:string
http://purl.uniprot.org/citations/19133617http://purl.uniprot.org/core/pages"425-431"xsd:string
http://purl.uniprot.org/citations/19133617http://purl.uniprot.org/core/pages"425-431"xsd:string
http://purl.uniprot.org/citations/19133617http://purl.uniprot.org/core/title"Detection and molecular characterization of enteroviruses in Korean surface water by using integrated cell culture multiplex RT-PCR."xsd:string
http://purl.uniprot.org/citations/19133617http://purl.uniprot.org/core/title"Detection and molecular characterization of enteroviruses in Korean surface water by using integrated cell culture multiplex RT-PCR."xsd:string
http://purl.uniprot.org/citations/19133617http://purl.uniprot.org/core/volume"21"xsd:string
http://purl.uniprot.org/citations/19133617http://purl.uniprot.org/core/volume"21"xsd:string
http://purl.uniprot.org/citations/19133617http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/19133617
http://purl.uniprot.org/citations/19133617http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/19133617
http://purl.uniprot.org/citations/19133617http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/19133617
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