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http://purl.uniprot.org/citations/19134217http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/19134217http://www.w3.org/2000/01/rdf-schema#comment"

Background

With current treatment strategies, nearly half of all medulloblastoma (MB) patients die from progressive tumors. Accordingly, the identification of novel therapeutic strategies remains a major goal. Deregulation of c-MYC is evident in numerous human cancers. In MB, over-expression of c-MYC has been shown to cause anaplasia and correlate with unfavorable prognosis.

Methods

To study the role of c-MYC in MB biology, we down-regulated c-MYC expression by using small interfering RNA (siRNA) and investigated changes in cellular proliferation, cell cycle analysis, apoptosis, telomere maintenance, and response to ionizing radiation (IR) and chemotherapeutics in a representative panel of human MB cell lines expressing different levels of c-MYC (DAOY wild-type, DAOY transfected with the empty vector, DAOY transfected with c-MYC, D341, and D425).

Results

siRNA-mediated c-MYC down-regulation resulted in an inhibition of cellular proliferation and clonogenic growth, inhibition of G1-S phase cell cycle progression, and a decrease in human telomerase reverse transcriptase (hTERT) expression and telomerase activity. On the other hand, down-regulation of c-MYC reduced apoptosis and decreased the sensitivity of human MB cells to IR, cisplatin, and etoposide. This effect was more pronounced in DAOY cells expressing high levels of c-MYC when compared with DAOY wild-type or DAOY cells transfected with the empty vector.

Conclusion

In human MB cells, in addition to its roles in growth and proliferation, c-MYC is also a potent inducer of apoptosis. Therefore, targeting c-MYC might be of therapeutic benefit when used sequentially with chemo- and radiotherapy rather than concomitantly."xsd:string
http://purl.uniprot.org/citations/19134217http://purl.org/dc/terms/identifier"doi:10.1186/1471-2407-9-10"xsd:string
http://purl.uniprot.org/citations/19134217http://purl.uniprot.org/core/author"Arnold L."xsd:string
http://purl.uniprot.org/citations/19134217http://purl.uniprot.org/core/author"Pruschy M."xsd:string
http://purl.uniprot.org/citations/19134217http://purl.uniprot.org/core/author"Eberhart C.G."xsd:string
http://purl.uniprot.org/citations/19134217http://purl.uniprot.org/core/author"Arcaro A."xsd:string
http://purl.uniprot.org/citations/19134217http://purl.uniprot.org/core/author"Grotzer M.A."xsd:string
http://purl.uniprot.org/citations/19134217http://purl.uniprot.org/core/author"Shalaby T."xsd:string
http://purl.uniprot.org/citations/19134217http://purl.uniprot.org/core/author"Stearns D."xsd:string
http://purl.uniprot.org/citations/19134217http://purl.uniprot.org/core/author"von Bueren A.O."xsd:string
http://purl.uniprot.org/citations/19134217http://purl.uniprot.org/core/author"Oehler-Janne C."xsd:string
http://purl.uniprot.org/citations/19134217http://purl.uniprot.org/core/date"2009"xsd:gYear
http://purl.uniprot.org/citations/19134217http://purl.uniprot.org/core/name"BMC Cancer"xsd:string
http://purl.uniprot.org/citations/19134217http://purl.uniprot.org/core/pages"10"xsd:string
http://purl.uniprot.org/citations/19134217http://purl.uniprot.org/core/title"RNA interference-mediated c-MYC inhibition prevents cell growth and decreases sensitivity to radio- and chemotherapy in childhood medulloblastoma cells."xsd:string
http://purl.uniprot.org/citations/19134217http://purl.uniprot.org/core/volume"9"xsd:string
http://purl.uniprot.org/citations/19134217http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/19134217
http://purl.uniprot.org/citations/19134217http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/19134217
http://purl.uniprot.org/uniprot/#_A0A0B4J1R1-mappedCitation-19134217http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19134217
http://purl.uniprot.org/uniprot/#_A0N2G3-mappedCitation-19134217http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19134217
http://purl.uniprot.org/uniprot/#_A0A224B085-mappedCitation-19134217http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19134217
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http://purl.uniprot.org/uniprot/#_B3CJ87-mappedCitation-19134217http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19134217
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