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http://purl.uniprot.org/citations/19141058http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/19141058http://www.w3.org/2000/01/rdf-schema#comment"

Background

In this study, we analyzed the gene expression profile of fibroblast-like synoviocyte (FLS) cultures from the temporomandibular joint (TMJ) to identify candidate genes associated with intracapsular pathologic conditions of TMJ. Cyclooxygenase (COX)-2 was one of the genes in FLS upregulated following stimulation by interleukin (IL)-1beta, a cytokine thought to play a key role in several pathological conditions. This study investigated the expression of COX-1 and COX-2 in cultured human FLS and rat TMJ synovium following stimulation with IL-1beta.

Methods

RNA was isolated from human FLS after IL-1beta treatment. COX-1 and -2 expression was examined using a GeneChip and real-time polymerase chain reaction. Prostaglandin E(2) (PGE(2)) levels in conditioned media from FLS were measured using enzyme-linked immunosorbent assay. Synovial tissues from TMJs of IL-1beta-injected rats were examined for COX-1 and COX-2 expression by immunohistochemical staining.

Results

Following treatment of FLS with IL-1beta, expression of the COX-2 gene increased up to 8 h and peaked at 4 h, whereas COX-1 expression did not change. Stimulation with IL-1beta increased the level of PGE(2) in conditioned media of cultured FLS in a time-dependent manner up to 48 h. Immunohistochemistry showed a strong positive staining for COX-2 in the lining and sub-lining synovial tissues of the TMJ of IL-1beta-injected rats. In contrast, staining for COX-1 was the same in synovial tissues with and without IL-1beta injection.

Conclusion

These data suggest that COX-2 expression stimulated by IL-1beta stimulates the production of PGE(2) in FLS and plays important roles in the progression of inflammation in TMJ."xsd:string
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http://purl.uniprot.org/citations/19141058http://purl.uniprot.org/core/author"Satoh K."xsd:string
http://purl.uniprot.org/citations/19141058http://purl.uniprot.org/core/author"Yamamoto H."xsd:string
http://purl.uniprot.org/citations/19141058http://purl.uniprot.org/core/author"Ogura N."xsd:string
http://purl.uniprot.org/citations/19141058http://purl.uniprot.org/core/author"Akutsu M."xsd:string
http://purl.uniprot.org/citations/19141058http://purl.uniprot.org/core/author"Kuyama K."xsd:string
http://purl.uniprot.org/citations/19141058http://purl.uniprot.org/core/author"Kondoh T."xsd:string
http://purl.uniprot.org/citations/19141058http://purl.uniprot.org/core/author"Kuboyama N."xsd:string
http://purl.uniprot.org/citations/19141058http://purl.uniprot.org/core/date"2009"xsd:gYear
http://purl.uniprot.org/citations/19141058http://purl.uniprot.org/core/name"J Oral Pathol Med"xsd:string
http://purl.uniprot.org/citations/19141058http://purl.uniprot.org/core/pages"584-590"xsd:string
http://purl.uniprot.org/citations/19141058http://purl.uniprot.org/core/title"Expression of cyclooxygenase-1 and -2 in IL-1beta-induced synovitis of the temporomandibular joint."xsd:string
http://purl.uniprot.org/citations/19141058http://purl.uniprot.org/core/volume"38"xsd:string
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