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http://purl.uniprot.org/citations/1915359http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/1915359http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/1915359http://www.w3.org/2000/01/rdf-schema#comment"The lux genes required for light expression in the luminescent bacterium Photobacterium leiognathi (ATCC 25521) have been cloned and expressed in Escherichia coli and their organization and nucleotide sequence determined. Transformation of a recombinant 9.5-kbp chromosomal DNA fragment of P. leiognathi into an E. coli mutant (43R) gave luminescent colonies that were as bright as those of the parental strain. Moreover, expression of the lux genes in the mutant E. coli was strong enough so that not only were high levels of luciferase detected in crude extracts, but the fatty-acid reductase activity responsible for synthesis of the aldehyde substrate for the luminescent reaction could readily be measured. Determination of the 7.3-kbp nucleotide sequence of P. leiognathi DNA, including the genes for luciferase (luxAB) and fatty-acid reductase (luxCDE) as well as a new lux gene (luxG) found recently in luminescent Vibrio species, showed that the order of the lux genes was luxCDABEG. Moreover, luxF, a gene homologous to luxB and located between luxB and luxE in Photobacterium but not Vibrio strains, was absent. In spite of this different lux gene organization, an intergenic stem-loop structure between luxB and luxE was discovered to be highly conserved in other Photobacterium species after luxF."xsd:string
http://purl.uniprot.org/citations/1915359http://purl.org/dc/terms/identifier"doi:10.1111/j.1432-1033.1991.tb16269.x"xsd:string
http://purl.uniprot.org/citations/1915359http://purl.org/dc/terms/identifier"doi:10.1111/j.1432-1033.1991.tb16269.x"xsd:string
http://purl.uniprot.org/citations/1915359http://purl.uniprot.org/core/author"Lee C.Y."xsd:string
http://purl.uniprot.org/citations/1915359http://purl.uniprot.org/core/author"Lee C.Y."xsd:string
http://purl.uniprot.org/citations/1915359http://purl.uniprot.org/core/author"Meighen E.A."xsd:string
http://purl.uniprot.org/citations/1915359http://purl.uniprot.org/core/author"Meighen E.A."xsd:string
http://purl.uniprot.org/citations/1915359http://purl.uniprot.org/core/author"Szittner R.B."xsd:string
http://purl.uniprot.org/citations/1915359http://purl.uniprot.org/core/author"Szittner R.B."xsd:string
http://purl.uniprot.org/citations/1915359http://purl.uniprot.org/core/date"1991"xsd:gYear
http://purl.uniprot.org/citations/1915359http://purl.uniprot.org/core/date"1991"xsd:gYear
http://purl.uniprot.org/citations/1915359http://purl.uniprot.org/core/name"Eur. J. Biochem."xsd:string
http://purl.uniprot.org/citations/1915359http://purl.uniprot.org/core/name"Eur. J. Biochem."xsd:string
http://purl.uniprot.org/citations/1915359http://purl.uniprot.org/core/pages"161-167"xsd:string
http://purl.uniprot.org/citations/1915359http://purl.uniprot.org/core/pages"161-167"xsd:string
http://purl.uniprot.org/citations/1915359http://purl.uniprot.org/core/title"The lux genes of the luminous bacterial symbiont, Photobacterium leiognathi, of the ponyfish. Nucleotide sequence, difference in gene organization, and high expression in mutant Escherichia coli."xsd:string
http://purl.uniprot.org/citations/1915359http://purl.uniprot.org/core/title"The lux genes of the luminous bacterial symbiont, Photobacterium leiognathi, of the ponyfish. Nucleotide sequence, difference in gene organization, and high expression in mutant Escherichia coli."xsd:string
http://purl.uniprot.org/citations/1915359http://purl.uniprot.org/core/volume"201"xsd:string
http://purl.uniprot.org/citations/1915359http://purl.uniprot.org/core/volume"201"xsd:string
http://purl.uniprot.org/citations/1915359http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/1915359
http://purl.uniprot.org/citations/1915359http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/1915359
http://purl.uniprot.org/citations/1915359http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/1915359
http://purl.uniprot.org/citations/1915359http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/1915359