| http://purl.uniprot.org/citations/19159283 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19159283 | http://www.w3.org/2000/01/rdf-schema#comment | "Physical interactions between proteins play a key role in probably every cellular process. Efforts to chart the protein interaction networks are ongoing in a number of model organisms using a diversity of approaches. The resulting genome-wide interaction maps will provide a scaffold for further detailed functional analysis. We developed MAPPIT, a mammalian two-hybrid approach that allows identification and analysis of mammalian protein-protein interactions in their native environment. Here, we introduce an efficient MAPPIT assay that permits high-throughput screening of arrayed collections of proteins and complements a previously published cDNA library screening approach. We validated both methods in screens for interaction partners of the Cullin-based E3 ubiquitin ligase subunits SKP1 and Elongin C. In addition to a number of known interactors, novel SKP1 and Elongin C binding proteins were identified. The array assay is an important addition to the MAPPIT suite of technologies that is expected to significantly increase its utility as a toolbox to screen for novel interactors of proteins or small molecules."xsd:string |
| http://purl.uniprot.org/citations/19159283 | http://purl.org/dc/terms/identifier | "doi:10.1021/pr8005167"xsd:string |
| http://purl.uniprot.org/citations/19159283 | http://purl.uniprot.org/core/author | "Vidal M."xsd:string |
| http://purl.uniprot.org/citations/19159283 | http://purl.uniprot.org/core/author | "Gesellchen V."xsd:string |
| http://purl.uniprot.org/citations/19159283 | http://purl.uniprot.org/core/author | "Tavernier J."xsd:string |
| http://purl.uniprot.org/citations/19159283 | http://purl.uniprot.org/core/author | "Lievens S."xsd:string |
| http://purl.uniprot.org/citations/19159283 | http://purl.uniprot.org/core/author | "Van der Heyden J."xsd:string |
| http://purl.uniprot.org/citations/19159283 | http://purl.uniprot.org/core/author | "Vanderroost N."xsd:string |
| http://purl.uniprot.org/citations/19159283 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19159283 | http://purl.uniprot.org/core/name | "J Proteome Res"xsd:string |
| http://purl.uniprot.org/citations/19159283 | http://purl.uniprot.org/core/pages | "877-886"xsd:string |
| http://purl.uniprot.org/citations/19159283 | http://purl.uniprot.org/core/title | "Array MAPPIT: high-throughput interactome analysis in mammalian cells."xsd:string |
| http://purl.uniprot.org/citations/19159283 | http://purl.uniprot.org/core/volume | "8"xsd:string |
| http://purl.uniprot.org/citations/19159283 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19159283 |
| http://purl.uniprot.org/citations/19159283 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19159283 |
| http://purl.uniprot.org/uniprot/#_Q15369-mappedCitation-19159283 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19159283 |
| http://purl.uniprot.org/uniprot/#_Q8N531-mappedCitation-19159283 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19159283 |
| http://purl.uniprot.org/uniprot/#_O14508-mappedCitation-19159283 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19159283 |
| http://purl.uniprot.org/uniprot/#_P83940-mappedCitation-19159283 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19159283 |
| http://purl.uniprot.org/uniprot/#_P63208-mappedCitation-19159283 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19159283 |
| http://purl.uniprot.org/uniprot/#_Q9H4M3-mappedCitation-19159283 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19159283 |
| http://purl.uniprot.org/uniprot/#_Q5XUX1-mappedCitation-19159283 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19159283 |
| http://purl.uniprot.org/uniprot/#_Q96DX5-mappedCitation-19159283 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19159283 |
| http://purl.uniprot.org/uniprot/#_Q6PJ61-mappedCitation-19159283 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19159283 |