| http://purl.uniprot.org/citations/19162005 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19162005 | http://www.w3.org/2000/01/rdf-schema#comment | "Proliferation of vascular endothelial cells (EC) and smooth muscle cells (SMC) is a critical event in angiogenesis and atherosclerosis. We previously showed that the C5b-9 assembly during complement activation induces cell cycle in human aortic EC (AEC) and SMC. C5b-9 can induce the expression of Response Gene to Complement (RGC)-32 and over expression of this gene leads to cell cycle activation. Therefore, the present study was carried out to test the requirement of endogenous RGC-32 for the cell cycle activation induced by C5b-9 by knocking-down its expression using siRNA. We identified two RGC-32 siRNAs that can markedly reduce the expression of RGC-32 mRNA in AEC. RGC-32 silencing in these cells abolished DNA synthesis induced by C5b-9 and serum growth factors, indicating the requirement of RGC-32 activity for S-phase entry. RGC-32 siRNA knockdown also significantly reduced the C5b-9 induced CDC2 activation and Akt phosphorylation. CDC2 does not play a role in G1/S transition in HeLa cells stably overexpressing RGC-32. RGC-32 was found to physically associate with Akt and was phosphorylated by Akt in vitro. Mutation of RGC-32 protein at Ser 45 and Ser 47 prevented Akt mediated phosphorylation. In addition, RGC-32 was found to regulate the release of growth factors from AEC. All these data together suggest that cell cycle induction by C5b-9 in AEC is RGC-32 dependent and this is in part through regulation of Akt and growth factor release."xsd:string |
| http://purl.uniprot.org/citations/19162005 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.yexmp.2008.12.005"xsd:string |
| http://purl.uniprot.org/citations/19162005 | http://purl.uniprot.org/core/author | "Ito T."xsd:string |
| http://purl.uniprot.org/citations/19162005 | http://purl.uniprot.org/core/author | "Fosbrink M."xsd:string |
| http://purl.uniprot.org/citations/19162005 | http://purl.uniprot.org/core/author | "Rus H."xsd:string |
| http://purl.uniprot.org/citations/19162005 | http://purl.uniprot.org/core/author | "Cudrici C."xsd:string |
| http://purl.uniprot.org/citations/19162005 | http://purl.uniprot.org/core/author | "Niculescu F."xsd:string |
| http://purl.uniprot.org/citations/19162005 | http://purl.uniprot.org/core/author | "Rus V."xsd:string |
| http://purl.uniprot.org/citations/19162005 | http://purl.uniprot.org/core/author | "Soloviova K."xsd:string |
| http://purl.uniprot.org/citations/19162005 | http://purl.uniprot.org/core/author | "Tegla C.A."xsd:string |
| http://purl.uniprot.org/citations/19162005 | http://purl.uniprot.org/core/author | "Vlaicu S."xsd:string |
| http://purl.uniprot.org/citations/19162005 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19162005 | http://purl.uniprot.org/core/name | "Exp Mol Pathol"xsd:string |
| http://purl.uniprot.org/citations/19162005 | http://purl.uniprot.org/core/pages | "87-94"xsd:string |
| http://purl.uniprot.org/citations/19162005 | http://purl.uniprot.org/core/title | "Response gene to complement 32 is required for C5b-9 induced cell cycle activation in endothelial cells."xsd:string |
| http://purl.uniprot.org/citations/19162005 | http://purl.uniprot.org/core/volume | "86"xsd:string |
| http://purl.uniprot.org/citations/19162005 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19162005 |
| http://purl.uniprot.org/citations/19162005 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19162005 |
| http://purl.uniprot.org/uniprot/Q9H4X1#attribution-CB5122812101602B65CE18708B222591 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/19162005 |
| http://purl.uniprot.org/uniprot/#_A0A8Q3SID6-mappedCitation-19162005 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19162005 |
| http://purl.uniprot.org/uniprot/#_A0A8Q3WL56-mappedCitation-19162005 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19162005 |
| http://purl.uniprot.org/uniprot/#_A8K8Z4-mappedCitation-19162005 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19162005 |
| http://purl.uniprot.org/uniprot/#_B2R6W1-mappedCitation-19162005 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19162005 |
| http://purl.uniprot.org/uniprot/#_A8K9M5-mappedCitation-19162005 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19162005 |