| http://purl.uniprot.org/citations/19172743 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19172743 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19172743 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/19172743 | http://www.w3.org/2000/01/rdf-schema#comment | "EF4 (LepA) is an almost universally conserved translational GTPase in eubacteria. It seems to be essential under environmental stress conditions and has previously been shown to back-translocate the tRNAs on the ribosome, thereby reverting the canonical translocation reaction. In the current work, EF4 was directly visualized in the process of back-translocating tRNAs by single-particle cryo-EM. Using flexible fitting methods, we built a model of ribosome-bound EF4 based on the cryo-EM map and a recently published unbound EF4 X-ray structure. The cryo-EM map establishes EF4 as a noncanonical elongation factor that interacts not only with the elongating ribosome, but also with the back-translocated tRNA in the A-site region, which is present in a previously unseen, intermediate state and deviates markedly from the position of a canonical A-tRNA. Our results, therefore, provide insight into the underlying structural principles governing back-translocation."xsd:string |
| http://purl.uniprot.org/citations/19172743 | http://purl.org/dc/terms/identifier | "doi:10.1038/nsmb.1469"xsd:string |
| http://purl.uniprot.org/citations/19172743 | http://purl.org/dc/terms/identifier | "doi:10.1038/nsmb.1469"xsd:string |
| http://purl.uniprot.org/citations/19172743 | http://purl.uniprot.org/core/author | "Fucini P."xsd:string |
| http://purl.uniprot.org/citations/19172743 | http://purl.uniprot.org/core/author | "Fucini P."xsd:string |
| http://purl.uniprot.org/citations/19172743 | http://purl.uniprot.org/core/author | "Nierhaus K.H."xsd:string |
| http://purl.uniprot.org/citations/19172743 | http://purl.uniprot.org/core/author | "Nierhaus K.H."xsd:string |
| http://purl.uniprot.org/citations/19172743 | http://purl.uniprot.org/core/author | "Wilson D.N."xsd:string |
| http://purl.uniprot.org/citations/19172743 | http://purl.uniprot.org/core/author | "Wilson D.N."xsd:string |
| http://purl.uniprot.org/citations/19172743 | http://purl.uniprot.org/core/author | "Qin Y."xsd:string |
| http://purl.uniprot.org/citations/19172743 | http://purl.uniprot.org/core/author | "Qin Y."xsd:string |
| http://purl.uniprot.org/citations/19172743 | http://purl.uniprot.org/core/author | "Topf M."xsd:string |
| http://purl.uniprot.org/citations/19172743 | http://purl.uniprot.org/core/author | "Topf M."xsd:string |
| http://purl.uniprot.org/citations/19172743 | http://purl.uniprot.org/core/author | "Mielke T."xsd:string |
| http://purl.uniprot.org/citations/19172743 | http://purl.uniprot.org/core/author | "Mielke T."xsd:string |
| http://purl.uniprot.org/citations/19172743 | http://purl.uniprot.org/core/author | "Spahn C.M."xsd:string |
| http://purl.uniprot.org/citations/19172743 | http://purl.uniprot.org/core/author | "Spahn C.M."xsd:string |
| http://purl.uniprot.org/citations/19172743 | http://purl.uniprot.org/core/author | "Connell S.R."xsd:string |
| http://purl.uniprot.org/citations/19172743 | http://purl.uniprot.org/core/author | "Connell S.R."xsd:string |
| http://purl.uniprot.org/citations/19172743 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/19172743 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/19172743 | http://purl.uniprot.org/core/name | "Nat. Struct. Mol. Biol."xsd:string |