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http://purl.uniprot.org/citations/19180498http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/19180498http://www.w3.org/2000/01/rdf-schema#comment"

Objective

To investigate the role of interleukin-22 (IL-22) in collagen-induced arthritis (CIA), an animal model of rheumatoid arthritis.

Methods

C57BL/6 mice were immunized with type II collagen (CII) in Freund's incomplete adjuvant with added Mycobacterium tuberculosis, and levels of IL-22 and its specific receptor, IL-22 receptor type I (IL-22RI), were measured in sera and tissue by enzyme-linked immunosorbent assay and real-time quantitative polymerase chain reaction analysis. Clinical and histologic signs of arthritis were recorded and compared with those in C57BL/6 mice deficient in the IL-22 gene (IL-22(-/-)). Humoral and cellular immune responses against CII were analyzed. In vitro osteoclastogenesis assays were performed on splenocytes.

Results

Upon immunization with CII in Freund's incomplete adjuvant plus heat-killed Mycobacterium tuberculosis, sera from C57BL/6 mice were found to contain high levels of IL-22, and the specific IL-22RI was expressed in lymphoid tissue, including splenocytes. IL-22(-/-) mice were less susceptible to CIA than were wild-type mice, as evidenced by their decreased incidence of arthritis and decreased pannus formation. Remarkably, the less severe form of arthritis in IL-22(-/-) mice was associated with increased production of CII-specific and total IgG antibodies, whereas cellular CII responses were unchanged. In vitro, IL-22 was found to promote osteoclastogenesis, a process that might contribute to its proinflammatory activity in CIA.

Conclusion

Endogenous IL-22 plays a proinflammatory role in CIA in C57BL/6 mice. Our data also indicate that IL-22 promotes osteoclastogenesis and regulates antibody production."xsd:string
http://purl.uniprot.org/citations/19180498http://purl.org/dc/terms/identifier"doi:10.1002/art.24220"xsd:string
http://purl.uniprot.org/citations/19180498http://purl.uniprot.org/core/author"Mitera T."xsd:string
http://purl.uniprot.org/citations/19180498http://purl.uniprot.org/core/author"Renauld J.C."xsd:string
http://purl.uniprot.org/citations/19180498http://purl.uniprot.org/core/author"Dumoutier L."xsd:string
http://purl.uniprot.org/citations/19180498http://purl.uniprot.org/core/author"Matthys P."xsd:string
http://purl.uniprot.org/citations/19180498http://purl.uniprot.org/core/author"Kelchtermans H."xsd:string
http://purl.uniprot.org/citations/19180498http://purl.uniprot.org/core/author"Geboes L."xsd:string
http://purl.uniprot.org/citations/19180498http://purl.uniprot.org/core/author"Schurgers E."xsd:string
http://purl.uniprot.org/citations/19180498http://purl.uniprot.org/core/date"2009"xsd:gYear
http://purl.uniprot.org/citations/19180498http://purl.uniprot.org/core/name"Arthritis Rheum"xsd:string
http://purl.uniprot.org/citations/19180498http://purl.uniprot.org/core/pages"390-395"xsd:string
http://purl.uniprot.org/citations/19180498http://purl.uniprot.org/core/title"Proinflammatory role of the Th17 cytokine interleukin-22 in collagen-induced arthritis in C57BL/6 mice."xsd:string
http://purl.uniprot.org/citations/19180498http://purl.uniprot.org/core/volume"60"xsd:string
http://purl.uniprot.org/citations/19180498http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/19180498
http://purl.uniprot.org/citations/19180498http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/19180498
http://purl.uniprot.org/uniprot/#_A0A7R8GV04-mappedCitation-19180498http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19180498
http://purl.uniprot.org/uniprot/#_Q3SXE1-mappedCitation-19180498http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19180498
http://purl.uniprot.org/uniprot/#_Q9JJY9-mappedCitation-19180498http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19180498
http://purl.uniprot.org/uniprot/A0A7R8GV04http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/19180498
http://purl.uniprot.org/uniprot/Q3SXE1http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/19180498
http://purl.uniprot.org/uniprot/Q9JJY9http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/19180498