| http://purl.uniprot.org/citations/19202133 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19202133 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19202133 | http://www.w3.org/2000/01/rdf-schema#comment | "The mammalian Delta6-desaturase coded by fatty acid desaturase 2 (FADS2; HSA11q12-q13.1) catalyzes the first and rate-limiting step for the biosynthesis of long-chain polyunsaturated fatty acids. FADS2 is known to act on at least five substrates, and we hypothesized that the FADS2 gene product would have Delta8-desaturase activity. Saccharomyces cerevisiae transformed with a FADS2 construct from baboon neonate liver cDNA gained the function to desaturate 11,14-eicosadienoic acid (20:2n-6) and 11,14,17-eicosatrienoic acid (20:3n-3) to yield 20:3n-6 and 20:4n-3, respectively. Competition experiments indicate that Delta8-desaturation favors activity toward 20:3n-3 over 20:2n-6 by 3-fold. Similar experiments show that Delta6-desaturase activity is favored over Delta8-desaturase activity by 7-fold and 23-fold for n-6 (18:2n-6 vs 20:2n-6) and n-3 (18:3n-3 vs 20:3n-3), respectively. In mammals, 20:3n-6 is the immediate precursor of prostaglandin E1 and thromboxane B1. 20:3n-6 and 20:4n-3 are also immediate precursors of long-chain polyunsaturated fatty acids arachidonic acid and eicosapentaenoic acid, respectively. These findings provide unequivocal molecular evidence for a novel alternative biosynthetic route to long-chain polyunsaturated fatty acids in mammals from substrates previously considered to be dead-end products."xsd:string |
| http://purl.uniprot.org/citations/19202133 | http://purl.org/dc/terms/identifier | "doi:10.1194/jlr.m800630-jlr200"xsd:string |
| http://purl.uniprot.org/citations/19202133 | http://purl.org/dc/terms/identifier | "doi:10.1194/jlr.m800630-jlr200"xsd:string |
| http://purl.uniprot.org/citations/19202133 | http://purl.uniprot.org/core/author | "Brenna J.T."xsd:string |
| http://purl.uniprot.org/citations/19202133 | http://purl.uniprot.org/core/author | "Brenna J.T."xsd:string |
| http://purl.uniprot.org/citations/19202133 | http://purl.uniprot.org/core/author | "Lawrence P."xsd:string |
| http://purl.uniprot.org/citations/19202133 | http://purl.uniprot.org/core/author | "Lawrence P."xsd:string |
| http://purl.uniprot.org/citations/19202133 | http://purl.uniprot.org/core/author | "Park W.J."xsd:string |
| http://purl.uniprot.org/citations/19202133 | http://purl.uniprot.org/core/author | "Park W.J."xsd:string |
| http://purl.uniprot.org/citations/19202133 | http://purl.uniprot.org/core/author | "Kothapalli K.S."xsd:string |
| http://purl.uniprot.org/citations/19202133 | http://purl.uniprot.org/core/author | "Kothapalli K.S."xsd:string |
| http://purl.uniprot.org/citations/19202133 | http://purl.uniprot.org/core/author | "Tyburczy C."xsd:string |
| http://purl.uniprot.org/citations/19202133 | http://purl.uniprot.org/core/author | "Tyburczy C."xsd:string |
| http://purl.uniprot.org/citations/19202133 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19202133 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19202133 | http://purl.uniprot.org/core/name | "J. Lipid Res."xsd:string |
| http://purl.uniprot.org/citations/19202133 | http://purl.uniprot.org/core/name | "J. Lipid Res."xsd:string |
| http://purl.uniprot.org/citations/19202133 | http://purl.uniprot.org/core/pages | "1195-1202"xsd:string |
| http://purl.uniprot.org/citations/19202133 | http://purl.uniprot.org/core/pages | "1195-1202"xsd:string |
| http://purl.uniprot.org/citations/19202133 | http://purl.uniprot.org/core/title | "An alternate pathway to long-chain polyunsaturates: the FADS2 gene product Delta8-desaturates 20:2n-6 and 20:3n-3."xsd:string |
| http://purl.uniprot.org/citations/19202133 | http://purl.uniprot.org/core/title | "An alternate pathway to long-chain polyunsaturates: the FADS2 gene product Delta8-desaturates 20:2n-6 and 20:3n-3."xsd:string |
| http://purl.uniprot.org/citations/19202133 | http://purl.uniprot.org/core/volume | "50"xsd:string |
| http://purl.uniprot.org/citations/19202133 | http://purl.uniprot.org/core/volume | "50"xsd:string |