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http://purl.uniprot.org/citations/19211270http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/19211270http://www.w3.org/2000/01/rdf-schema#comment"

Background

The arterial vasculature is subjected to considerably greater biomechanical stress than the venous circulation. This is reflected in the difference in morphology between large arteries and veins, however little is known about the molecular differences that arise as a consequence of biomechanical stress. Previously, we identified a group of arterial intima-enriched (AIE) genes: sciellin, periplakin, SPRR3, envoplakin, galectin 7, and plakoglobin that are functionally related in that they contribute to the stress properties of stratified epithelium. We sought to test our hypothesis that these genes were regulated by biomechanical stress in vascular smooth muscle cells (VSMCs).

Methods

Immunofluorescence was employed to determine the expression of the AIE genes in saphenous vein coronary artery bypass grafts. Furthermore, we used a model of cyclic stress to determine if the AIE genes were regulated by biomechanical stress in VSMCs in vitro.

Results

Sciellin and periplakin were upregulated in saphenous vein coronary artery bypass grafts after arterialization, but were absent in non-arterialized saphenous veins. Sciellin, SPRR3, and periplakin transcripts were all upregulated (4.67-, 4.95-, 2.77-fold, respectively) by prolonged exposure to cyclic strain (24-72 h), but not at earlier time points.

Conclusions

These findings suggest a novel role for several human AIE genes in the VSMC response to arterialization and extended cyclic strain.

Summary

Biomechanical stress has long been implicated in vascular pathologies. We report the novel finding of a group of genes, previously studied in stratified epithelium, that were regulated by prolonged cyclic stress in vascular smooth muscle cells. This may have important implications to vascular disease."xsd:string
http://purl.uniprot.org/citations/19211270http://purl.org/dc/terms/identifier"doi:10.1016/j.carpath.2008.12.006"xsd:string
http://purl.uniprot.org/citations/19211270http://purl.uniprot.org/core/author"Li B."xsd:string
http://purl.uniprot.org/citations/19211270http://purl.uniprot.org/core/author"Olson S."xsd:string
http://purl.uniprot.org/citations/19211270http://purl.uniprot.org/core/author"Crimmins D.L."xsd:string
http://purl.uniprot.org/citations/19211270http://purl.uniprot.org/core/author"Atkinson J.B."xsd:string
http://purl.uniprot.org/citations/19211270http://purl.uniprot.org/core/author"Maupin A.B."xsd:string
http://purl.uniprot.org/citations/19211270http://purl.uniprot.org/core/author"Young P.P."xsd:string
http://purl.uniprot.org/citations/19211270http://purl.uniprot.org/core/author"Pyle A.L."xsd:string
http://purl.uniprot.org/citations/19211270http://purl.uniprot.org/core/author"Guzman R.J."xsd:string
http://purl.uniprot.org/citations/19211270http://purl.uniprot.org/core/date"2010"xsd:gYear
http://purl.uniprot.org/citations/19211270http://purl.uniprot.org/core/name"Cardiovasc Pathol"xsd:string
http://purl.uniprot.org/citations/19211270http://purl.uniprot.org/core/pages"e13-20"xsd:string
http://purl.uniprot.org/citations/19211270http://purl.uniprot.org/core/title"Biomechanical stress induces novel arterial intima-enriched genes: implications for vascular adaptation to stress."xsd:string
http://purl.uniprot.org/citations/19211270http://purl.uniprot.org/core/volume"19"xsd:string
http://purl.uniprot.org/citations/19211270http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/19211270
http://purl.uniprot.org/citations/19211270http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/19211270
http://purl.uniprot.org/uniprot/O60437#attribution-F16F5D275FCE9C67C2A43CA38A27D3FBhttp://purl.uniprot.org/core/sourcehttp://purl.uniprot.org/citations/19211270
http://purl.uniprot.org/uniprot/O95171#attribution-91CE872AC94EBF94BA9BF0FDBFFB9B86http://purl.uniprot.org/core/sourcehttp://purl.uniprot.org/citations/19211270
http://purl.uniprot.org/uniprot/O95171#attribution-F16F5D275FCE9C67C2A43CA38A27D3FBhttp://purl.uniprot.org/core/sourcehttp://purl.uniprot.org/citations/19211270
http://purl.uniprot.org/uniprot/Q9UBC9#attribution-91CE872AC94EBF94BA9BF0FDBFFB9B86http://purl.uniprot.org/core/sourcehttp://purl.uniprot.org/citations/19211270
http://purl.uniprot.org/uniprot/#_B7Z394-mappedCitation-19211270http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19211270
http://purl.uniprot.org/uniprot/#_O60437-mappedCitation-19211270http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19211270
http://purl.uniprot.org/uniprot/#_O95171-mappedCitation-19211270http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19211270
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