Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/19218102http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/19218102http://www.w3.org/2000/01/rdf-schema#comment"

Objective

To investigate the effect of angiotensin II type-1 (AT-1) alpha receptor gene silencing on nuclear factor-kappaB (NF-kappaB) activity in hepatic Kupffer cells.

Methods

The expression of AT-1 alpha receptors in primary isolated cultured hepatic Kupffer cells was detected by immunohistochemistry. pSilencer/AT-1 alpha receptor siRNA plasmids were transfected into Kupffer cells, which were subsequently exposed to 10(-6) mol/L angiotensin II (Ang II) for 60 min. The changes in the DNA binding activity of NF-kappaB in the cells was assessed using electrophoretic gel mobility shift assay (EMSA).

Results

AT-1 alpha receptor expression was detected in Kupffer cells. NF-kappaB DNA binding activity was markedly increased in Kupffer cells after Ang II stimulation, and obviously inhibited by transfectiom with pSilencer/AT-1 alpha receptor siRNA plasmid.

Conclusion

Ang II stimulation of Kupffer cell results in increased activation of NF-kappaB via AT-1 alpha receptor."xsd:string
http://purl.uniprot.org/citations/19218102http://purl.uniprot.org/core/author"Li X."xsd:string
http://purl.uniprot.org/citations/19218102http://purl.uniprot.org/core/author"Meng Y."xsd:string
http://purl.uniprot.org/citations/19218102http://purl.uniprot.org/core/author"Zhang Z.S."xsd:string
http://purl.uniprot.org/citations/19218102http://purl.uniprot.org/core/author"Zhou G.S."xsd:string
http://purl.uniprot.org/citations/19218102http://purl.uniprot.org/core/date"2009"xsd:gYear
http://purl.uniprot.org/citations/19218102http://purl.uniprot.org/core/name"Nan Fang Yi Ke Da Xue Xue Bao"xsd:string
http://purl.uniprot.org/citations/19218102http://purl.uniprot.org/core/pages"20-22"xsd:string
http://purl.uniprot.org/citations/19218102http://purl.uniprot.org/core/title"[Effect of AT-1 alpha receptor gene silencing on nuclear factor-kappaB activity in hepatic Kupffer cells]."xsd:string
http://purl.uniprot.org/citations/19218102http://purl.uniprot.org/core/volume"29"xsd:string
http://purl.uniprot.org/citations/19218102http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/19218102
http://purl.uniprot.org/citations/19218102http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/19218102
http://purl.uniprot.org/uniprot/#_A0A0A0MSE3-mappedCitation-19218102http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19218102
http://purl.uniprot.org/uniprot/#_A0A510GAH8-mappedCitation-19218102http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19218102
http://purl.uniprot.org/uniprot/#_D3DNG8-mappedCitation-19218102http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19218102
http://purl.uniprot.org/uniprot/#_Q13063-mappedCitation-19218102http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19218102
http://purl.uniprot.org/uniprot/#_P30556-mappedCitation-19218102http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19218102
http://purl.uniprot.org/uniprot/#_Q53YY0-mappedCitation-19218102http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19218102
http://purl.uniprot.org/uniprot/#_P19838-mappedCitation-19218102http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19218102
http://purl.uniprot.org/uniprot/#_Q6NUP5-mappedCitation-19218102http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19218102
http://purl.uniprot.org/uniprot/P19838http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/19218102
http://purl.uniprot.org/uniprot/A0A510GAH8http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/19218102
http://purl.uniprot.org/uniprot/Q6NUP5http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/19218102
http://purl.uniprot.org/uniprot/A0A0A0MSE3http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/19218102
▶