| http://purl.uniprot.org/citations/19218499 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19218499 | http://www.w3.org/2000/01/rdf-schema#comment | "Diet-induced weight loss is affected by a wide range of factors, including genetic variation. Identifying functional polymorphisms will help to elucidate mechanisms that account for variation in dietary metabolism. Previously, we reported a strong association between a common single nucleotide polymorphism (SNP) rs2419621 (C>T) in the promoter of acyl-CoA synthetase long chain 5 (ACSL5), rapid weight loss in obese Caucasian females, and elevated ACSL5 mRNA levels in skeletal muscle biopsies. Here, we showed by electrophoretic mobility shift assay (EMSA) that the T allele creates a functional cis-regulatory E-box element (CANNTG) that is recognized by the myogenic regulatory factor MyoD. The T allele promoted MyoD-dependent activation of a 1089-base pair ACSL5 promoter fragment in nonmuscle CV1 cells. Differentiation of skeletal myoblasts significantly elevated expression of the ACSL5 promoter. The T allele sustained promoter activity 48 h after differentiation, whereas the C allele showed a significant decline. These results reveal a mechanism for elevated transcription of ACSL5 in skeletal muscle of carriers of the rs2419621(T) allele, associated with more rapid diet-induced weight loss. Natural selection favoring promoter polymorphisms that reduced expression of catabolic genes in skeletal muscle likely accounts for the resistance of obese individuals to dietary intervention."xsd:string |
| http://purl.uniprot.org/citations/19218499 | http://purl.org/dc/terms/identifier | "doi:10.1096/fj.08-120998"xsd:string |
| http://purl.uniprot.org/citations/19218499 | http://purl.uniprot.org/core/author | "Stewart A.F."xsd:string |
| http://purl.uniprot.org/citations/19218499 | http://purl.uniprot.org/core/author | "Teng A.C."xsd:string |
| http://purl.uniprot.org/citations/19218499 | http://purl.uniprot.org/core/author | "Tesson F."xsd:string |
| http://purl.uniprot.org/citations/19218499 | http://purl.uniprot.org/core/author | "Adamo K."xsd:string |
| http://purl.uniprot.org/citations/19218499 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19218499 | http://purl.uniprot.org/core/name | "FASEB J"xsd:string |
| http://purl.uniprot.org/citations/19218499 | http://purl.uniprot.org/core/pages | "1705-1709"xsd:string |
| http://purl.uniprot.org/citations/19218499 | http://purl.uniprot.org/core/title | "Functional characterization of a promoter polymorphism that drives ACSL5 gene expression in skeletal muscle and associates with diet-induced weight loss."xsd:string |
| http://purl.uniprot.org/citations/19218499 | http://purl.uniprot.org/core/volume | "23"xsd:string |
| http://purl.uniprot.org/citations/19218499 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19218499 |
| http://purl.uniprot.org/citations/19218499 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19218499 |
| http://purl.uniprot.org/uniprot/#_B4DX30-mappedCitation-19218499 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19218499 |
| http://purl.uniprot.org/uniprot/#_Q9ULC5-mappedCitation-19218499 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19218499 |
| http://purl.uniprot.org/uniprot/Q9ULC5 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/19218499 |
| http://purl.uniprot.org/uniprot/B4DX30 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/19218499 |