| http://purl.uniprot.org/citations/19220816 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19220816 | http://www.w3.org/2000/01/rdf-schema#comment | "Subcellular compartmentalization of exoribonucleases (RNAses) is an important control mechanism in the temporal and spatial regulation of RNA processing and decay. Despite much progress towards understanding RNAse substrates and functions, we know little of how RNAses are transported and assembled into functional, subcellularly restricted complexes. To gain insight into this issue, we are studying the exosome-binding protein Dis3, a processive 3' to 5' exoribonuclease. Here, we examine the interactions and subcellular localization of the Drosophila melanogaster Dis3 (dDis3) protein. N-terminal domain mutants of dDis3 abolish associations with the 'core' exosome, yet only reduce binding to the 'nuclear' exosome-associated factor dRrp6. We show that nuclear localization of dDis3 requires a C-terminal classic nuclear localization signal (NLS). Consistent with this, dDis3 specifically co-precipitates the NLS-binding protein importin-alpha3. Surprisingly, dDis3 constructs that lack or mutate the C-terminal NLS retain importin-alpha3 binding, suggesting that the interaction is indirect. Finally, we find that endogenous dDis3 and dRrp6 exhibit coordinated nuclear enrichment or exclusion, suggesting that dDis3, Rrp6 and importin-alpha3 interact in a complex independent of the core. We propose that the movement and deposition of this complex is important for the subcellular compartmentalization and regulation of the exosome core."xsd:string |
| http://purl.uniprot.org/citations/19220816 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1600-0854.2009.00888.x"xsd:string |
| http://purl.uniprot.org/citations/19220816 | http://purl.uniprot.org/core/author | "Andrulis E.D."xsd:string |
| http://purl.uniprot.org/citations/19220816 | http://purl.uniprot.org/core/author | "Graham A.C."xsd:string |
| http://purl.uniprot.org/citations/19220816 | http://purl.uniprot.org/core/author | "Davis S.M."xsd:string |
| http://purl.uniprot.org/citations/19220816 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19220816 | http://purl.uniprot.org/core/name | "Traffic"xsd:string |
| http://purl.uniprot.org/citations/19220816 | http://purl.uniprot.org/core/pages | "499-513"xsd:string |
| http://purl.uniprot.org/citations/19220816 | http://purl.uniprot.org/core/title | "Interdependent nucleocytoplasmic trafficking and interactions of Dis3 with Rrp6, the core exosome and importin-alpha3."xsd:string |
| http://purl.uniprot.org/citations/19220816 | http://purl.uniprot.org/core/volume | "10"xsd:string |
| http://purl.uniprot.org/citations/19220816 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19220816 |
| http://purl.uniprot.org/citations/19220816 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19220816 |
| http://purl.uniprot.org/uniprot/#_A4V4A5-mappedCitation-19220816 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19220816 |
| http://purl.uniprot.org/uniprot/#_O18388-mappedCitation-19220816 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19220816 |
| http://purl.uniprot.org/uniprot/#_P52295-mappedCitation-19220816 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19220816 |
| http://purl.uniprot.org/uniprot/#_Q9BI20-mappedCitation-19220816 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19220816 |
| http://purl.uniprot.org/uniprot/#_M9NDC3-mappedCitation-19220816 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19220816 |
| http://purl.uniprot.org/uniprot/#_O76521-mappedCitation-19220816 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19220816 |
| http://purl.uniprot.org/uniprot/#_O76522-mappedCitation-19220816 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19220816 |
| http://purl.uniprot.org/uniprot/#_Q29QQ9-mappedCitation-19220816 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19220816 |
| http://purl.uniprot.org/uniprot/#_Q5BID5-mappedCitation-19220816 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19220816 |
| http://purl.uniprot.org/uniprot/#_Q5U119-mappedCitation-19220816 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19220816 |
| http://purl.uniprot.org/uniprot/#_Q95TW1-mappedCitation-19220816 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19220816 |
| http://purl.uniprot.org/uniprot/#_Q960A7-mappedCitation-19220816 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19220816 |