| http://purl.uniprot.org/citations/19223394 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19223394 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19223394 | http://www.w3.org/2000/01/rdf-schema#comment | "The covalent attachment of small ubiquitin-like modifier (SUMO) proteins regulates protein localization and function. SUMOylation has recently been shown to modulate ion-channel function; however, the extent to which this affects native currents and cellular excitability remains to be determined. The voltage-dependent K(+) (Kv) channel Kv2.1 regulates pancreatic beta-cell excitability and insulin secretion. We found that YFP-tagged SUMO1 (SUMO1-YFP) can be immunoprecipitated with Kv2.1 when these two proteins are coexpressed in HEK 293 cells. Furthermore, direct infusion of recombinant SUMO1 peptide or coexpression of SUMO1-YFP inhibited current through cloned Kv2.1 by 80% and 48%, respectively. Insulin-secreting cells express SUMO variants 1 and 3, and expression of the SUMO1-YFP in human beta-cells or insulinoma cells inhibited native Kv currents (by 49% and 33%, respectively). Inhibition of the channel resulted from an acceleration of channel inactivation and an inhibition of recovery from inactivation, resulting in the widening of beta-cell action potentials and a decreased firing frequency. Finally, these effects on channel function and excitability were augmented by the conjugating enzyme Ubc9 and rescued by the SUMO protease SENP1. Thus, protein SUMOylation can exert a strong inhibitory action on the voltage-dependent K(+) channel Kv2.1 and can regulate cellular excitability in native beta-cells."xsd:string |
| http://purl.uniprot.org/citations/19223394 | http://purl.org/dc/terms/identifier | "doi:10.1242/jcs.036632"xsd:string |
| http://purl.uniprot.org/citations/19223394 | http://purl.org/dc/terms/identifier | "doi:10.1242/jcs.036632"xsd:string |
| http://purl.uniprot.org/citations/19223394 | http://purl.uniprot.org/core/author | "Dai X.Q."xsd:string |
| http://purl.uniprot.org/citations/19223394 | http://purl.uniprot.org/core/author | "Dai X.Q."xsd:string |
| http://purl.uniprot.org/citations/19223394 | http://purl.uniprot.org/core/author | "Sipione S."xsd:string |
| http://purl.uniprot.org/citations/19223394 | http://purl.uniprot.org/core/author | "Sipione S."xsd:string |
| http://purl.uniprot.org/citations/19223394 | http://purl.uniprot.org/core/author | "Kolic J."xsd:string |
| http://purl.uniprot.org/citations/19223394 | http://purl.uniprot.org/core/author | "Kolic J."xsd:string |
| http://purl.uniprot.org/citations/19223394 | http://purl.uniprot.org/core/author | "Macdonald P.E."xsd:string |
| http://purl.uniprot.org/citations/19223394 | http://purl.uniprot.org/core/author | "Macdonald P.E."xsd:string |
| http://purl.uniprot.org/citations/19223394 | http://purl.uniprot.org/core/author | "Marchi P."xsd:string |
| http://purl.uniprot.org/citations/19223394 | http://purl.uniprot.org/core/author | "Marchi P."xsd:string |
| http://purl.uniprot.org/citations/19223394 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19223394 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19223394 | http://purl.uniprot.org/core/name | "J. Cell Sci."xsd:string |
| http://purl.uniprot.org/citations/19223394 | http://purl.uniprot.org/core/name | "J. Cell Sci."xsd:string |
| http://purl.uniprot.org/citations/19223394 | http://purl.uniprot.org/core/pages | "775-779"xsd:string |
| http://purl.uniprot.org/citations/19223394 | http://purl.uniprot.org/core/pages | "775-779"xsd:string |
| http://purl.uniprot.org/citations/19223394 | http://purl.uniprot.org/core/title | "SUMOylation regulates Kv2.1 and modulates pancreatic beta-cell excitability."xsd:string |
| http://purl.uniprot.org/citations/19223394 | http://purl.uniprot.org/core/title | "SUMOylation regulates Kv2.1 and modulates pancreatic beta-cell excitability."xsd:string |
| http://purl.uniprot.org/citations/19223394 | http://purl.uniprot.org/core/volume | "122"xsd:string |
| http://purl.uniprot.org/citations/19223394 | http://purl.uniprot.org/core/volume | "122"xsd:string |