| http://purl.uniprot.org/citations/19232093 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19232093 | http://www.w3.org/2000/01/rdf-schema#comment | "BackgroundDuring apoptosis cells become profoundly restructured through concerted cleavage of cellular proteins by caspases. In epithelial tissues, apoptotic cells loose their apical/basal polarity and are extruded from the epithelium. We used the Drosophila embryo as a system to investigate the regulation of components of the zonula adherens during apoptosis. Since Armadillo/beta-catenin (Arm) is a major regulator of cadherin-mediated adhesion, we analyzed the mechanisms of Arm proteolysis in apoptosis.ResultsWe define early and late apoptotic stages and find that early in apoptosis Dalpha-catenin remains relatively stable, while Arm and DE-cadherin protein levels are strongly reduced. Arm is cleaved by caspases in embryo extracts and we provide evidence that the caspase-3 homolog drICE cleaves Arm in vitro and in vivo. Cleavage by drICE creates a stable protein fragment that remains associated with the plasma membrane early in apoptosis. To further understand the role of caspase-mediated cleavage of Arm, we examined potential caspase cleavage sites and found that drICE cleaves Arm at a unique DQVD motif in the N-terminal domain of the protein. Mutation of the drICE cleavage site in Arm results in a protein that is not cleaved in vitro and in vivo. Furthermore we provide evidence that cleavage of Arm plays a role in the removal of DE-cadherin from the plasma membrane during apoptosis.ConclusionThis study defines the specificity of caspase cleavage of Arm in Drosophila apoptotic cells. Our data suggest that N-terminal truncation of Arm by caspases is evolutionarily conserved and thus might provide a principal mechanism involved in the disassembly of adherens junctions during apoptosis."xsd:string |
| http://purl.uniprot.org/citations/19232093 | http://purl.org/dc/terms/identifier | "doi:10.1186/1471-213x-9-15"xsd:string |
| http://purl.uniprot.org/citations/19232093 | http://purl.uniprot.org/core/author | "Kessler T."xsd:string |
| http://purl.uniprot.org/citations/19232093 | http://purl.uniprot.org/core/author | "Muller H.A."xsd:string |
| http://purl.uniprot.org/citations/19232093 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19232093 | http://purl.uniprot.org/core/name | "BMC Dev Biol"xsd:string |
| http://purl.uniprot.org/citations/19232093 | http://purl.uniprot.org/core/pages | "15"xsd:string |
| http://purl.uniprot.org/citations/19232093 | http://purl.uniprot.org/core/title | "Cleavage of Armadillo/beta-catenin by the caspase DrICE in Drosophila apoptotic epithelial cells."xsd:string |
| http://purl.uniprot.org/citations/19232093 | http://purl.uniprot.org/core/volume | "9"xsd:string |
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