| http://purl.uniprot.org/citations/19250909 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19250909 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19250909 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/19250909 | http://www.w3.org/2000/01/rdf-schema#comment | "Ubiquitin and ubiquitin-like proteins (UBLs) are directed to targets by cascades of E1, E2, and E3 enzymes. The largest ubiquitin E3 subclass consists of cullin-RING ligases (CRLs), which contain one each of several cullins (CUL1, -2, -3, -4, or -5) and RING proteins (RBX1 or -2). CRLs are activated by ligation of the UBL NEDD8 to a conserved cullin lysine. How is cullin NEDD8ylation specificity established? Here we report that, like UBE2M (also known as UBC12), the previously uncharacterized E2 UBE2F is a NEDD8-conjugating enzyme in vitro and in vivo. Biochemical and structural analyses indicate how plasticity of hydrophobic E1-E2 interactions and E1 conformational flexibility allow one E1 to charge multiple E2s. The E2s have distinct functions, with UBE2M/RBX1 and UBE2F/RBX2 displaying different target cullin specificities. Together, these studies reveal the molecular basis for and functional importance of hierarchical expansion of the NEDD8 conjugation system in establishing selective CRL activation."xsd:string |
| http://purl.uniprot.org/citations/19250909 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.molcel.2009.01.011"xsd:string |
| http://purl.uniprot.org/citations/19250909 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.molcel.2009.01.011"xsd:string |
| http://purl.uniprot.org/citations/19250909 | http://purl.uniprot.org/core/author | "Schulman B.A."xsd:string |
| http://purl.uniprot.org/citations/19250909 | http://purl.uniprot.org/core/author | "Schulman B.A."xsd:string |
| http://purl.uniprot.org/citations/19250909 | http://purl.uniprot.org/core/author | "Neale G."xsd:string |
| http://purl.uniprot.org/citations/19250909 | http://purl.uniprot.org/core/author | "Neale G."xsd:string |
| http://purl.uniprot.org/citations/19250909 | http://purl.uniprot.org/core/author | "Scott D.C."xsd:string |
| http://purl.uniprot.org/citations/19250909 | http://purl.uniprot.org/core/author | "Scott D.C."xsd:string |
| http://purl.uniprot.org/citations/19250909 | http://purl.uniprot.org/core/author | "Duda D.M."xsd:string |
| http://purl.uniprot.org/citations/19250909 | http://purl.uniprot.org/core/author | "Duda D.M."xsd:string |
| http://purl.uniprot.org/citations/19250909 | http://purl.uniprot.org/core/author | "Taherbhoy A.M."xsd:string |
| http://purl.uniprot.org/citations/19250909 | http://purl.uniprot.org/core/author | "Taherbhoy A.M."xsd:string |
| http://purl.uniprot.org/citations/19250909 | http://purl.uniprot.org/core/author | "Ayrault O."xsd:string |
| http://purl.uniprot.org/citations/19250909 | http://purl.uniprot.org/core/author | "Ayrault O."xsd:string |
| http://purl.uniprot.org/citations/19250909 | http://purl.uniprot.org/core/author | "Murray P.J."xsd:string |
| http://purl.uniprot.org/citations/19250909 | http://purl.uniprot.org/core/author | "Murray P.J."xsd:string |
| http://purl.uniprot.org/citations/19250909 | http://purl.uniprot.org/core/author | "Roussel M.F."xsd:string |
| http://purl.uniprot.org/citations/19250909 | http://purl.uniprot.org/core/author | "Roussel M.F."xsd:string |
| http://purl.uniprot.org/citations/19250909 | http://purl.uniprot.org/core/author | "Borg L.A."xsd:string |
| http://purl.uniprot.org/citations/19250909 | http://purl.uniprot.org/core/author | "Borg L.A."xsd:string |
| http://purl.uniprot.org/citations/19250909 | http://purl.uniprot.org/core/author | "Hunt H.W."xsd:string |