| http://purl.uniprot.org/citations/19261606 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19261606 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19261606 | http://www.w3.org/2000/01/rdf-schema#comment | "The proper regulation of factors involved in mitosis is crucial to ensure normal cell division. Levels and activities of proteins are regulated in many ways, one of which is ubiquitin-mediated protein degradation. E3 ubiquitin ligases are involved in targeting specific substrates for degradation by facilitating their ubiquitination. In seeking to elucidate additional biological roles for Cul3 we performed a two-hybrid screen and identified Ctb9/KLHDC5 as a Cul3-interacting protein. Overexpression of Ctb9/KLHDC5 resulted in an increase in microtubule density as well as persistent microtubule bridges between post-mitotic cells. Conversely, down-regulation of Ctb9/KLHDC5 showed a pronounced reduction in microtubule density. Based on these observations, we examined the interactions between Cul3, Ctb9/KLHDC5, and the microtubule-severing protein, p60/katanin. Here we show that p60/katanin interacts with a complex consisting of Cul3 and Ctb9/KLHDC5, which results in ubiquitin laddering of p60/katanin. Also, Cul3-deficient cells or Ctb9/KLHDC5-deficient cells show an increase in p60/katanin levels, indicating that Cul3/Ctb9/KLHDC5 is required for efficient p60/katanin removal. We demonstrate a novel regulatory mechanism for p60/katanin that occurs at the level of targeted proteolysis to allow normal mitotic progression in mammalian cells."xsd:string |
| http://purl.uniprot.org/citations/19261606 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m809374200"xsd:string |
| http://purl.uniprot.org/citations/19261606 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m809374200"xsd:string |
| http://purl.uniprot.org/citations/19261606 | http://purl.uniprot.org/core/author | "Singer J.D."xsd:string |
| http://purl.uniprot.org/citations/19261606 | http://purl.uniprot.org/core/author | "Singer J.D."xsd:string |
| http://purl.uniprot.org/citations/19261606 | http://purl.uniprot.org/core/author | "Perdue S.A."xsd:string |
| http://purl.uniprot.org/citations/19261606 | http://purl.uniprot.org/core/author | "Perdue S.A."xsd:string |
| http://purl.uniprot.org/citations/19261606 | http://purl.uniprot.org/core/author | "Bentley C.A."xsd:string |
| http://purl.uniprot.org/citations/19261606 | http://purl.uniprot.org/core/author | "Bentley C.A."xsd:string |
| http://purl.uniprot.org/citations/19261606 | http://purl.uniprot.org/core/author | "Baas P.W."xsd:string |
| http://purl.uniprot.org/citations/19261606 | http://purl.uniprot.org/core/author | "Baas P.W."xsd:string |
| http://purl.uniprot.org/citations/19261606 | http://purl.uniprot.org/core/author | "Cummings C.M."xsd:string |
| http://purl.uniprot.org/citations/19261606 | http://purl.uniprot.org/core/author | "Cummings C.M."xsd:string |
| http://purl.uniprot.org/citations/19261606 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19261606 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19261606 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/19261606 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/19261606 | http://purl.uniprot.org/core/pages | "11663-11675"xsd:string |
| http://purl.uniprot.org/citations/19261606 | http://purl.uniprot.org/core/pages | "11663-11675"xsd:string |
| http://purl.uniprot.org/citations/19261606 | http://purl.uniprot.org/core/title | "The Cul3/Klhdc5 E3 ligase regulates p60/katanin and is required for normal mitosis in mammalian cells."xsd:string |
| http://purl.uniprot.org/citations/19261606 | http://purl.uniprot.org/core/title | "The Cul3/Klhdc5 E3 ligase regulates p60/katanin and is required for normal mitosis in mammalian cells."xsd:string |
| http://purl.uniprot.org/citations/19261606 | http://purl.uniprot.org/core/volume | "284"xsd:string |
| http://purl.uniprot.org/citations/19261606 | http://purl.uniprot.org/core/volume | "284"xsd:string |