| http://purl.uniprot.org/citations/19264809 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19264809 | http://www.w3.org/2000/01/rdf-schema#comment | "7,8-Dihydro-8-oxoguanine (8-oxoG) is an abundant and mutagenic DNA lesion. In Saccharomyces cerevisiae, the 8-oxoG DNA N-glycosylase (Ogg1) acts as the primary defense against 8-oxoG. Here, we present evidence for cooperation between Rad18-Rad6-dependent monoubiquitylation of PCNA at K164, the damage-tolerant DNA polymerase eta and the mismatch repair system (MMR) to prevent 8-oxoG-induced mutagenesis. Preventing PCNA modification at lysine 164 (pol30-K164R) results in a dramatic increase in GC to TA mutations due to endogenous 8-oxoG in Ogg1-deficient cells. In contrast, deletion of RAD5 or SIZ1 has little effect implying that the modification of PCNA relevant for preventing 8-oxoG-induced mutagenesis is monoubiquitin as opposed to polyubiquitin or SUMO. We also report that the ubiquitin-binding domain (UBZ) of Pol eta is essential to prevent 8-oxoG-induced mutagenesis but only in conjunction with a functional PCNA-binding domain (PIP). We propose that PCNA is ubiquitylated during the repair synthesis reaction after the MMR-dependent excision of adenine incorporated opposite to 8-oxoG. Monoubiquitylation of PCNA would favor the recruitment of Pol eta thereby allowing error-free incorporation of dCMP opposite to 8-oxoG. This study suggests that Pol eta and the post-replication repair (PRR) machinery can also prevent mutagenesis at DNA lesions that do not stall replication forks."xsd:string |
| http://purl.uniprot.org/citations/19264809 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/gkp105"xsd:string |
| http://purl.uniprot.org/citations/19264809 | http://purl.uniprot.org/core/author | "Boiteux S."xsd:string |
| http://purl.uniprot.org/citations/19264809 | http://purl.uniprot.org/core/author | "Ulrich H.D."xsd:string |
| http://purl.uniprot.org/citations/19264809 | http://purl.uniprot.org/core/author | "van der Kemp P.A."xsd:string |
| http://purl.uniprot.org/citations/19264809 | http://purl.uniprot.org/core/author | "de Padula M."xsd:string |
| http://purl.uniprot.org/citations/19264809 | http://purl.uniprot.org/core/author | "Burguiere-Slezak G."xsd:string |
| http://purl.uniprot.org/citations/19264809 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19264809 | http://purl.uniprot.org/core/name | "Nucleic Acids Res"xsd:string |
| http://purl.uniprot.org/citations/19264809 | http://purl.uniprot.org/core/pages | "2549-2559"xsd:string |
| http://purl.uniprot.org/citations/19264809 | http://purl.uniprot.org/core/title | "PCNA monoubiquitylation and DNA polymerase eta ubiquitin-binding domain are required to prevent 8-oxoguanine-induced mutagenesis in Saccharomyces cerevisiae."xsd:string |
| http://purl.uniprot.org/citations/19264809 | http://purl.uniprot.org/core/volume | "37"xsd:string |
| http://purl.uniprot.org/citations/19264809 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19264809 |
| http://purl.uniprot.org/citations/19264809 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19264809 |
| http://purl.uniprot.org/uniprot/P15873#attribution-C89CCFA5FF589CAD955A62B8DFF0DBF1 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/19264809 |
| http://purl.uniprot.org/uniprot/P53397#attribution-C89CCFA5FF589CAD955A62B8DFF0DBF1 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/19264809 |
| http://purl.uniprot.org/uniprot/#_P15873-mappedCitation-19264809 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19264809 |
| http://purl.uniprot.org/uniprot/#_Q04049-mappedCitation-19264809 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19264809 |
| http://purl.uniprot.org/uniprot/P15873 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/19264809 |
| http://purl.uniprot.org/uniprot/Q04049 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/19264809 |