| http://purl.uniprot.org/citations/19265139 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19265139 | http://www.w3.org/2000/01/rdf-schema#comment | "To present virus and tumor Ags, HLA class I molecules undergo a complex multistep assembly involving discrete but transient folding intermediates. The most extensive folding abnormalities occur in cells lacking the class I L chain subunit, called beta(2)-microglobulin (beta(2)m). Herein, this issue was investigated taking advantage of eight conformational murine mAbs (including the prototypic W6/32 mAb) to mapped H chain epitopes of class I molecules, four human mAbs to class I alloantigens, as well as radioimmunoprecipitation, in vitro assembly, pulse-chase, flow cytometry, and peptide-pulse/ELISPOT experiments. We show that endogenous (HLA-A1, -A66, and -B58) as well as transfected (HLA-A2) heavy chains in beta(2)m-defective Burkitt lymphoma Daudi cells are capable of being expressed on the cell surface, although at low levels, and exclusively as immature glycoforms. In addition, HLA-A2 is: 1) partially folded at crucial interfaces with beta(2)m, peptide Ag, and CD8; 2) receptive to exogenous peptide; and 3) capable of presenting exogenous peptide epitopes (from virus and tumor Ags) to cytotoxic T lymphocytes (bulk populations as well as clones) educated in a beta(2)m-positive environment. These experiments demonstrate a precursor-product relationship between novel HLA class I folding intermediates, and define a stepwise mechanism whereby distinct interfaces of the class I H chain undergo successive, ligand-induced folding adjustments in vitro as well as in vivo. Due to this unprecedented class I plasticity, Daudi is the first human cell line in which folding and function of class I HLA molecules are observed in the absence of beta(2)m. These findings bear potential implications for tumor immunotherapy."xsd:string |
| http://purl.uniprot.org/citations/19265139 | http://purl.org/dc/terms/identifier | "doi:10.4049/jimmunol.0802316"xsd:string |
| http://purl.uniprot.org/citations/19265139 | http://purl.uniprot.org/core/author | "Mulder A."xsd:string |
| http://purl.uniprot.org/citations/19265139 | http://purl.uniprot.org/core/author | "Fruci D."xsd:string |
| http://purl.uniprot.org/citations/19265139 | http://purl.uniprot.org/core/author | "Giacomini P."xsd:string |
| http://purl.uniprot.org/citations/19265139 | http://purl.uniprot.org/core/author | "Sibilio L."xsd:string |
| http://purl.uniprot.org/citations/19265139 | http://purl.uniprot.org/core/author | "Lo Monaco E."xsd:string |
| http://purl.uniprot.org/citations/19265139 | http://purl.uniprot.org/core/author | "Martayan A."xsd:string |
| http://purl.uniprot.org/citations/19265139 | http://purl.uniprot.org/core/author | "Tremante E."xsd:string |
| http://purl.uniprot.org/citations/19265139 | http://purl.uniprot.org/core/author | "Rivoltini L."xsd:string |
| http://purl.uniprot.org/citations/19265139 | http://purl.uniprot.org/core/author | "Cova A."xsd:string |
| http://purl.uniprot.org/citations/19265139 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19265139 | http://purl.uniprot.org/core/name | "J Immunol"xsd:string |
| http://purl.uniprot.org/citations/19265139 | http://purl.uniprot.org/core/pages | "3609-3617"xsd:string |
| http://purl.uniprot.org/citations/19265139 | http://purl.uniprot.org/core/title | "Class I HLA folding and antigen presentation in beta 2-microglobulin-defective Daudi cells."xsd:string |
| http://purl.uniprot.org/citations/19265139 | http://purl.uniprot.org/core/volume | "182"xsd:string |
| http://purl.uniprot.org/citations/19265139 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19265139 |
| http://purl.uniprot.org/citations/19265139 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19265139 |
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