| http://purl.uniprot.org/citations/19298834 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19298834 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19298834 | http://www.w3.org/2000/01/rdf-schema#comment | "Antimicrobial peptides are components of innate immunity that is the first-line defense against invading pathogens for a wide range of organisms. Here, we describe the isolation, biological characterization and amino acid sequencing of a novel neutral Glycine/Leucine-rich antimicrobial peptide from skin secretion of Leptodactylus pentadactylus named leptoglycin. The amino acid sequence of the peptide purified by RP-HPLC (C(18) column) was deduced by mass spectrometric de novo sequencing and confirmed by Edman degradation: GLLGGLLGPLLGGGGGGGGGLL. Leptoglycin was able to inhibit the growth of Gram-negative bacteria Pseudomonas aeruginosa, Escherichia coli and Citrobacter freundii with minimal inhibitory concentrations (MICs) of 8 microM, 50 microM, and 75 microM respectively, but it did not show antimicrobial activity against Gram-positive bacteria (Staphylococcus aureus, Micrococcus luteus and Enterococcus faecalis), yeasts (Candida albicans and Candida tropicalis) and dermatophytes fungi (Microsporum canis and Trichophyton rubrum). No hemolytic activity was observed at the 2-200 microM range concentration. The amino acid sequence of leptoglycin with high level of glycine (59.1%) and leucine (36.4%) containing an unusual central proline suggests the existence of a new class of Gly/Leu-rich antimicrobial peptides. Taken together, these results suggest that this natural antimicrobial peptide could be a tool to develop new antibiotics."xsd:string |
| http://purl.uniprot.org/citations/19298834 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.toxicon.2009.03.011"xsd:string |
| http://purl.uniprot.org/citations/19298834 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.toxicon.2009.03.011"xsd:string |
| http://purl.uniprot.org/citations/19298834 | http://purl.uniprot.org/core/author | "Richardson M."xsd:string |
| http://purl.uniprot.org/citations/19298834 | http://purl.uniprot.org/core/author | "Richardson M."xsd:string |
| http://purl.uniprot.org/citations/19298834 | http://purl.uniprot.org/core/author | "Pimenta D.C."xsd:string |
| http://purl.uniprot.org/citations/19298834 | http://purl.uniprot.org/core/author | "Pimenta D.C."xsd:string |
| http://purl.uniprot.org/citations/19298834 | http://purl.uniprot.org/core/author | "Konno K."xsd:string |
| http://purl.uniprot.org/citations/19298834 | http://purl.uniprot.org/core/author | "Konno K."xsd:string |
| http://purl.uniprot.org/citations/19298834 | http://purl.uniprot.org/core/author | "Berto R.F."xsd:string |
| http://purl.uniprot.org/citations/19298834 | http://purl.uniprot.org/core/author | "Berto R.F."xsd:string |
| http://purl.uniprot.org/citations/19298834 | http://purl.uniprot.org/core/author | "Camargo A.A.C.M."xsd:string |
| http://purl.uniprot.org/citations/19298834 | http://purl.uniprot.org/core/author | "Camargo A.A.C.M."xsd:string |
| http://purl.uniprot.org/citations/19298834 | http://purl.uniprot.org/core/author | "Cardi B.A."xsd:string |
| http://purl.uniprot.org/citations/19298834 | http://purl.uniprot.org/core/author | "Cardi B.A."xsd:string |
| http://purl.uniprot.org/citations/19298834 | http://purl.uniprot.org/core/author | "Carvalho K.M."xsd:string |
| http://purl.uniprot.org/citations/19298834 | http://purl.uniprot.org/core/author | "Carvalho K.M."xsd:string |
| http://purl.uniprot.org/citations/19298834 | http://purl.uniprot.org/core/author | "Fontenele-Cardi N.C."xsd:string |
| http://purl.uniprot.org/citations/19298834 | http://purl.uniprot.org/core/author | "Fontenele-Cardi N.C."xsd:string |
| http://purl.uniprot.org/citations/19298834 | http://purl.uniprot.org/core/author | "Gois E.A."xsd:string |
| http://purl.uniprot.org/citations/19298834 | http://purl.uniprot.org/core/author | "Gois E.A."xsd:string |
| http://purl.uniprot.org/citations/19298834 | http://purl.uniprot.org/core/author | "Honorio-Junior J.E.R."xsd:string |
| http://purl.uniprot.org/citations/19298834 | http://purl.uniprot.org/core/author | "Honorio-Junior J.E.R."xsd:string |