| http://purl.uniprot.org/citations/19318419 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19318419 | http://www.w3.org/2000/01/rdf-schema#comment | "Cetuximab, an anti-epidermal growth factor receptor monoclonal antibody, has been shown to increase the median survival of colorectal cancer patients. We previously reported that the expression of HLA-E is significantly increased in primary human colorectal cancer, perhaps contributing to tumour escape from immune surveillance. To establish if HLA-E could be a factor that renders colorectal cancer cells less susceptible to antibody-dependent cellular cytotoxicity (ADCC), in the present study we analysed Cetuximab-mediated cytotoxicity against several colorectal cancer cell lines expressing, or not, HLA-E at the cell surface. We first observed that colorectal cancer cells treated with Cetuximab were killed more efficiently by ADCC. Interestingly, treatment of target cells with recombinant human-beta2-microglobulin inhibits Cetuximab-mediated ADCC through HLA-E membrane stabilization. The specific immunosuppressive role of HLA-E was confirmed using an anti-NKG2A monoclonal antibody, that restored the ability of immune cells to kill their target. This result demonstrates that HLA-E at the cell surface can reliably suppress the ADCC effect. On the other hand, Cetuximab induced a direct growth inhibition but only at high concentrations; furthermore, the CDC effect was quite moderate, and we failed to observe a pro-apoptotic effect. Taking into account that our findings suggest that ADCC activity is the main anti-tumour effect observed at clinically achievable concentrations of Cetuximab at the tumour site, we suggest that determination of HLA-E in colorectal cancer could be relevant to predict success of Cetuximab treatment."xsd:string |
| http://purl.uniprot.org/citations/19318419 | http://purl.org/dc/terms/identifier | "doi:10.1177/1753425908101404"xsd:string |
| http://purl.uniprot.org/citations/19318419 | http://purl.uniprot.org/core/author | "Gonzalez M."xsd:string |
| http://purl.uniprot.org/citations/19318419 | http://purl.uniprot.org/core/author | "Sycz G."xsd:string |
| http://purl.uniprot.org/citations/19318419 | http://purl.uniprot.org/core/author | "Bianchini M."xsd:string |
| http://purl.uniprot.org/citations/19318419 | http://purl.uniprot.org/core/author | "Mordoh J."xsd:string |
| http://purl.uniprot.org/citations/19318419 | http://purl.uniprot.org/core/author | "Barrio M.M."xsd:string |
| http://purl.uniprot.org/citations/19318419 | http://purl.uniprot.org/core/author | "Levy E.M."xsd:string |
| http://purl.uniprot.org/citations/19318419 | http://purl.uniprot.org/core/author | "von Euw E.M."xsd:string |
| http://purl.uniprot.org/citations/19318419 | http://purl.uniprot.org/core/author | "Arriaga J.M."xsd:string |
| http://purl.uniprot.org/citations/19318419 | http://purl.uniprot.org/core/author | "Morales S.B."xsd:string |
| http://purl.uniprot.org/citations/19318419 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19318419 | http://purl.uniprot.org/core/name | "Innate Immun"xsd:string |
| http://purl.uniprot.org/citations/19318419 | http://purl.uniprot.org/core/pages | "91-100"xsd:string |
| http://purl.uniprot.org/citations/19318419 | http://purl.uniprot.org/core/title | "Cetuximab-mediated cellular cytotoxicity is inhibited by HLA-E membrane expression in colon cancer cells."xsd:string |
| http://purl.uniprot.org/citations/19318419 | http://purl.uniprot.org/core/volume | "15"xsd:string |
| http://purl.uniprot.org/citations/19318419 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19318419 |
| http://purl.uniprot.org/citations/19318419 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19318419 |
| http://purl.uniprot.org/uniprot/#_A0A0F7WBW2-mappedCitation-19318419 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19318419 |
| http://purl.uniprot.org/uniprot/#_A0A0A8LFF7-mappedCitation-19318419 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19318419 |
| http://purl.uniprot.org/uniprot/#_A0A0F7W1Q1-mappedCitation-19318419 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19318419 |
| http://purl.uniprot.org/uniprot/#_P13747-mappedCitation-19318419 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19318419 |
| http://purl.uniprot.org/uniprot/#_A0A7L7TM74-mappedCitation-19318419 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19318419 |
| http://purl.uniprot.org/uniprot/#_A0A4E9DQ67-mappedCitation-19318419 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19318419 |