| http://purl.uniprot.org/citations/19338062 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19338062 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19338062 | http://www.w3.org/2000/01/rdf-schema#comment | "Bcps constitute a group of antioxidant enzymes, belonging to the Prx family, that are widely distributed in bacteria, plants, and fungi. These proteins can contain two conserved cysteines within the CXXXXC motif. Recent studies demonstrated that though the role of the first cysteine is well defined, being the catalytic peroxidatic cysteine in all the members of this protein family, data on the function of the second cysteine are controversial and require further investigation. In this article, we report on the functional and structural characterization of Bcp1, an archaeal Bcp isolated from Sulfolobus solfataricus, which presents two conserved cysteine residues at positions 45 and 50. Functional studies revealed that this enzyme performs the catalytic reaction using an atypical 2-Cys mechanism, where Cys45 is the peroxidatic and Cys50 is the resolving cysteine. The X-ray structure of the double mutant C45S/C50S, representative of the fully reduced enzyme state, was determined at a resolution of 2.15 A, showing a Trx fold similar to that of other Prxs. Superposition with a structural homologue in the oxidized state provided, for the first time, a detailed description of the structural rearrangement necessary for a member of the Bcp family to perform the catalytic reaction. From this structural analysis, it emerges that a significant conformational change from a fully folded, to a locally unfolded form is required to form the intramolecular disulfide bond upon oxidation, according to the proposed reaction mechanism. Two residues, namely Arg53 and Asp54, which could play a role in this rearrangement, were also identified."xsd:string |
| http://purl.uniprot.org/citations/19338062 | http://purl.org/dc/terms/identifier | "doi:10.1002/prot.22408"xsd:string |
| http://purl.uniprot.org/citations/19338062 | http://purl.org/dc/terms/identifier | "doi:10.1002/prot.22408"xsd:string |
| http://purl.uniprot.org/citations/19338062 | http://purl.uniprot.org/core/author | "Bartolucci S."xsd:string |
| http://purl.uniprot.org/citations/19338062 | http://purl.uniprot.org/core/author | "Bartolucci S."xsd:string |
| http://purl.uniprot.org/citations/19338062 | http://purl.uniprot.org/core/author | "De Simone G."xsd:string |
| http://purl.uniprot.org/citations/19338062 | http://purl.uniprot.org/core/author | "De Simone G."xsd:string |
| http://purl.uniprot.org/citations/19338062 | http://purl.uniprot.org/core/author | "Pedone C."xsd:string |
| http://purl.uniprot.org/citations/19338062 | http://purl.uniprot.org/core/author | "Pedone C."xsd:string |
| http://purl.uniprot.org/citations/19338062 | http://purl.uniprot.org/core/author | "Limauro D."xsd:string |
| http://purl.uniprot.org/citations/19338062 | http://purl.uniprot.org/core/author | "Limauro D."xsd:string |
| http://purl.uniprot.org/citations/19338062 | http://purl.uniprot.org/core/author | "D'Ambrosio K."xsd:string |
| http://purl.uniprot.org/citations/19338062 | http://purl.uniprot.org/core/author | "D'Ambrosio K."xsd:string |
| http://purl.uniprot.org/citations/19338062 | http://purl.uniprot.org/core/author | "Pedone E."xsd:string |
| http://purl.uniprot.org/citations/19338062 | http://purl.uniprot.org/core/author | "Pedone E."xsd:string |
| http://purl.uniprot.org/citations/19338062 | http://purl.uniprot.org/core/author | "Galdi I."xsd:string |
| http://purl.uniprot.org/citations/19338062 | http://purl.uniprot.org/core/author | "Galdi I."xsd:string |
| http://purl.uniprot.org/citations/19338062 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19338062 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19338062 | http://purl.uniprot.org/core/name | "Proteins"xsd:string |
| http://purl.uniprot.org/citations/19338062 | http://purl.uniprot.org/core/name | "Proteins"xsd:string |
| http://purl.uniprot.org/citations/19338062 | http://purl.uniprot.org/core/pages | "995-1006"xsd:string |
| http://purl.uniprot.org/citations/19338062 | http://purl.uniprot.org/core/pages | "995-1006"xsd:string |