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http://purl.uniprot.org/citations/19367720http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/19367720http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/19367720http://www.w3.org/2000/01/rdf-schema#comment"T lymphocytes mediate cellular and humoral defense against foreign bodies or autoantigens. An understanding of T-cell information processing furthers studies of the immunological response. We describe a large-scale phosphorylation analysis of primary T cells using a multidimensional separation strategy, involving preparative SDS-PAGE for prefractionation, in-gel digestion and sequential phosphopeptide enrichment using IMAC and TiO2. A total of 281 phosphorylation sites (197 of high confidence, Ascore > 15), mapping to 204 human gene sequences, were identified by LC-MS(n) analysis in an LTQ linear ion trap. Subsequently, we created the LymPHOS database (http://lymphos.org), which links mass spectrometric peptide information to phosphorylation sites and phosphoprotein sequences."xsd:string
http://purl.uniprot.org/citations/19367720http://purl.org/dc/terms/identifier"doi:10.1021/pr800500r"xsd:string
http://purl.uniprot.org/citations/19367720http://purl.org/dc/terms/identifier"doi:10.1021/pr800500r"xsd:string
http://purl.uniprot.org/citations/19367720http://purl.uniprot.org/core/author"Abian J."xsd:string
http://purl.uniprot.org/citations/19367720http://purl.uniprot.org/core/author"Abian J."xsd:string
http://purl.uniprot.org/citations/19367720http://purl.uniprot.org/core/author"Carrascal M."xsd:string
http://purl.uniprot.org/citations/19367720http://purl.uniprot.org/core/author"Carrascal M."xsd:string
http://purl.uniprot.org/citations/19367720http://purl.uniprot.org/core/author"Casas V."xsd:string
http://purl.uniprot.org/citations/19367720http://purl.uniprot.org/core/author"Casas V."xsd:string
http://purl.uniprot.org/citations/19367720http://purl.uniprot.org/core/author"Gay M."xsd:string
http://purl.uniprot.org/citations/19367720http://purl.uniprot.org/core/author"Gay M."xsd:string
http://purl.uniprot.org/citations/19367720http://purl.uniprot.org/core/author"Ovelleiro D."xsd:string
http://purl.uniprot.org/citations/19367720http://purl.uniprot.org/core/author"Ovelleiro D."xsd:string
http://purl.uniprot.org/citations/19367720http://purl.uniprot.org/core/date"2008"xsd:gYear
http://purl.uniprot.org/citations/19367720http://purl.uniprot.org/core/date"2008"xsd:gYear
http://purl.uniprot.org/citations/19367720http://purl.uniprot.org/core/name"J. Proteome Res."xsd:string
http://purl.uniprot.org/citations/19367720http://purl.uniprot.org/core/name"J. Proteome Res."xsd:string
http://purl.uniprot.org/citations/19367720http://purl.uniprot.org/core/pages"5167-5176"xsd:string
http://purl.uniprot.org/citations/19367720http://purl.uniprot.org/core/pages"5167-5176"xsd:string
http://purl.uniprot.org/citations/19367720http://purl.uniprot.org/core/title"Phosphorylation analysis of primary human T lymphocytes using sequential IMAC and titanium oxide enrichment."xsd:string
http://purl.uniprot.org/citations/19367720http://purl.uniprot.org/core/title"Phosphorylation analysis of primary human T lymphocytes using sequential IMAC and titanium oxide enrichment."xsd:string
http://purl.uniprot.org/citations/19367720http://purl.uniprot.org/core/volume"7"xsd:string
http://purl.uniprot.org/citations/19367720http://purl.uniprot.org/core/volume"7"xsd:string